SpringerProtocols: Abstract: 13. Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II

13. Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II

By: Grzegorz Jackowski²

Abstract

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The protocols regarding separation and purification of LHCII subcomplexes by non-denaturing isoelectric focusing (ndIEF), confirmation of trimeric nature of the subcomplexes, and establishing their polypeptide composition are described below.

Affiliation(s): (2) Department of Plant Physiology, Adam Mickiewicz University, Poznan, Poland

Book Title: Photosynthesis Research Protocols

Series: Methods in Molecular Biology | Volume: 274 | Pub. Date: Jun-08-2004 | Page Range: 115-128 | DOI: 10.1385/1-59259-799-8:115

Subject: Plant Sciences

Key Words: Circular dichroism - denaturing isoelectrofocusing - electrophoresis - immunoblotting - light-harvesting chlorophyll a/b-protein complex of photosystem II (LHCII) - monomer - nondenaturing isoelectrofocusing - polypeptide - stacked thylakoid membrane - subcomplex - trimer, Sultracentrifugation

References

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