SpringerProtocols: Abstract: Analysis of Proteins by CE, CIEF, and Microfluidic Devices With Whole-Column-Imaging Detection

Analysis of Proteins by CE, CIEF, and Microfluidic Devices With Whole-Column-Imaging Detection

By: Jiaqi Wu³, Xing-Zheng Wu⁴, Tiemin Huang⁵, Janusz Pawliszyn⁵

Abstract

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The recently developed whole-column-imaging detection technique for capillary electrophoresis (CE) and capillary isoelectric focusing (CIEF), a commercial whole-column-imaged CIEF instrument and its standard operation protocol are introduced. Furthermore, new developments and applications of whole-column-imaging detection in protein-protein interaction study, in protein separation using microfluidic devices and CIEF methods without carrier ampholytes, as well as in 2D separation techniques are reviewed. Miniaturization of whole-column-imaging CIEF and axially illuminated fluorescence whole-column-imaging CIEF are also discussed.

Affiliation(s): (3) Convergent Bioscience Ltd., Toronto, Ontario, Canada
(4) Department of Materials Science and Engineering, Fukui University, Fukui-shi, Japan
(5) Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada

Book Title: Capillary Electrophoresis of Proteins and Peptides

Series: Methods in Molecular Biology | Volume: 276 | Pub. Date: Jun-03-2004 | Page Range: 229-252 | DOI: 10.1385/1-59259-798-X:229

Subject: Biochemistry

Key Words: Capillary electrophoresis - capillary isoelectric focusing - conjugation reaction - microfluidic device - protein - whole-column-imaging detection

References

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