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Covalent and Noncovalent Labeling Schemes for Near-Infrared Dyes in Capillary Electrophoresis Protein Applications
| Abstract |
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Capillary electrophoresis (CE) is experiencing increased use in the field of separation science. Part of its growing popularity
of capillary electrophoresis can be attributed to the high efficiency of the separations achievable with the technique, making
it an attractive tool for bioanalytical applications. Laser-induced fluorescence (LIF) is a common detection method for CE.
One of the problems frequently experienced when using visible LIF detection is matrix autofluorescence which has the effect
of degrading the overall sensitivity of the technique. However, the use of near-infrared (NIR) laser induced fluorescence
nearly eliminates matrix autofluorescence, as very few molecules have intrinsic fluorescence in this region. This chapter
describes the use of covalent and noncovalent labeling schemes for tagging biomolecules with near infrared dyes. To fully
appreciate the advantages that the NIR LIF technique can supply, we also review applications that employ detection schemes
other than NIR LIF. Specific applications to be discussed include drug-protein studies by CE, as well as capillary electrophoretic
immunoassays.
Affiliation(s): (3) Department of Chemistry, Georgia State University, University Plaza, Atlanta, GA
Series: Methods in Molecular Biology | Volume: 276 | Pub. Date: Jun-03-2004 | Page Range: 39-75 | DOI: 10.1385/1-59259-798-X:039
Subject: Biochemistry
Key Words: Capillary electrophoresis - capillary electrophoretic immunoassay - covalent - drug binding constant - dye displacement - human serum albumin - labeling - laser-induced fluorescence - near infrared - noncovalent
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