SpringerProtocols: Abstract: Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo

Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo

By: Jae Woon Lee^{2 3}, Soo-Kyung Lee⁴

Abstract

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Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.

Affiliation(s): (2) Department of Medicine, Division of Diabetes, Endocrinology, and Metabolism, Baylor College of Medicine, Houston, TX
(3) Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX
(4) Gene Expression Laboratory, The Salk Institute, San Diego, CA

Book Title: Protein-Protein Interactions: Methods and Applications

Series: Methods in Molecular Biology | Volume: 261 | Pub. Date: Mar-23-2004 | Page Range: 327-336 | DOI: 10.1385/1-59259-762-9:327

Subject: Protein Science

Key Words: Chimera - Gal4 - VP16 - interaction - transcription

References

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