SpringerProtocols: Abstract: Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection

Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection

By: Stephen R. Ikeda²

Abstract

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Although methods for expressing foreign proteins in clonal cell lines are well established, mature neurons remain a difficult preparation for the introduction of foreign genes. Microinjection is a reliable method for producing robust targeted expression in neurons that has advantages over conventional transfection/infection methodologies. Here, I describe procedures for expressing signaling proteins in adult rat sympathetic neurons by direct microinjection of cRNA and cDNA into the cytoplasm and nucleus, respectively. The methods are applicable to a wide variety of peripheral and central neuron preparations, as well as clonal cell lines.

Affiliation(s): (2) Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD

Book Title: Receptor Signal Transduction Protocols

Series: Methods in Molecular Biology | Volume: 259 | Pub. Date: Mar-01-2004 | Page Range: 167-181 | DOI: 10.1385/1-59259-754-8:167

Subject: Cell Biology

Key Words: G-proteins - heterologous expression - ion channels - microinjection - sympathetic neurons - tissue culture

References

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