|
|
||
10. BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes
| Abstract |
|
|
Bacterial artificial chromosomes (BACs) have become a central tool in functional genomics. This is due to their average cloning
capacity (around 150 Kb), which can accommodate most eukaryotic genes along with their full set of regulatory elements, and
to their greater convenience in handling over other large cloning vectors like P1-based artificial chromosomes (PACs) and
yeast artificial chromosomes (YACs). Two key advances for harnessing the full power of BACs have been the development of methods
to modify them with precision and ease (see below), and the ability to use them for transgenesis in higher model organisms,
like mouse and zebrafish (for review, see (1)).
Affiliation(s): (3) BioInnovation Zentrum, University of Technology, Dresden, Germany
(4) c/o Max-Planck Institut for Cell Biology and Genetics, Dresden, Germany
(5) European Molecular Biology Laboratory, Heidelberg, Germany
(6) Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
(7) Gene Bridges GmbH, Dresden, Germany
(8) Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany
(9) Gene Expression Program, European Molecular Biology Laboratory, Heidelberg, Germany
(4) c/o Max-Planck Institut for Cell Biology and Genetics, Dresden, Germany
(5) European Molecular Biology Laboratory, Heidelberg, Germany
(6) Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
(7) Gene Bridges GmbH, Dresden, Germany
(8) Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany
(9) Gene Expression Program, European Molecular Biology Laboratory, Heidelberg, Germany
Series: Methods in Molecular Biology | Volume: 256 | Pub. Date: Mar-04-2004 | Page Range: 123-139 | DOI: 10.1385/1-59259-753-X:123
Subject: Microbiology
Comments (Loading...) |
||
 Loading... |