SpringerProtocols: Abstract: 2. Exon Trapping for Positional Cloning and Fingerprinting

2. Exon Trapping for Positional Cloning and Fingerprinting

By: Scott E. Wenderfer³, John J. Monaco⁴

Abstract

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Positional cloning involves the genetic, physical, and transcript mapping of specific parts of a genome (1). Linkage analysis can map specific activities, or phenotypes, to a quantitative trait locus (QTL), a genomic region no smaller than 1 centiMorgan (cM) or megabase (Mb) in length. Physical mapping can then provide a map of higher resolution. Physical maps are constructed from clones identified by screening genomic libraries. Genomic clones can be characterized by fingerprinting and ordered to create a contig, a contiguous array of overlapping clones. Transcript identification from the clones in the contig results in a map of genes within the physical map. Finally, expressional and functional studies must be performed to verify gene content.

Affiliation(s): (3) Department of Pediatrics, University of Texas Houston, Houston, TX
(4) Howard Hughes Medical Institute, University of Cincinnati, Cincinnati, OH

Book Title: Bacterial Artificial Chromosomes: Volume 2 Functional Studies

Series: Methods in Molecular Biology | Volume: 256 | Pub. Date: Mar-04-2004 | Page Range: 7-20 | DOI: 10.1385/1-59259-753-X:007

Subject: Microbiology

References

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