SpringerProtocols: Abstract: 8. Quantitative Analysis of the Usage of Human T-Cell Receptor α and β Chain Variable Regions by Reverse Dot-Blot Hybridization

8. Quantitative Analysis of the Usage of Human T-Cell Receptor α and β Chain Variable Regions by Reverse Dot-Blot Hybridization

By: Takaji Matsutani², Takeshi Yoshioka², Yuji Tsuruta², Shoji Iwagami², Tomoko Toyosaki-Maeda², Ryuji Suzuki²

Abstract

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Mature T lymphocytes specifically recognize antigens through interaction between MHC/peptide complex and αβ or γδ TCR. Variable region genes created by recombination of variable (V), diversity (D), and joining (J) locus contribute to shaping T cell receptor (TCR) repertoires (1,2). Thus, TCRαV and TCRβV repertoires have been studied for characterizing of T cells that may play major role in peripheral blood or pathological lesions of patients with autoimmune diseases (3–6).

Affiliation(s): (2) Department of Immunology, Shionogi Institute for Medical Science, Shionogi and Co. Ltd., Osaka, Japan

Book Title: T Cell Protocols: Development and Activation

Series: Methods in Molecular Biology | Volume: 134 | Pub. Date: Dec-22-1999 | Page Range: 81-101 | DOI: 10.1385/1-59259-682-7:81

Subject: Immunology

References

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