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6. Analysis of CD4/CD8 Lineage Commitment by Pronase Treatment and Reexpression Assay
Abstract
During the developmental process of T cells in the thymus, immature CD4CD8 double positive thymocytes (DP) are subject to undergo positive selection process to become either CD4 single positive (SP) or CD8-SP mature cells (13). Since both CD4-SP and CD8-SP mature T cells are the progeny of immature DP thymocytes, positive selection requires a lineage commitment step in which synthesis of one or the other coreceptor molecule is terminated. The mechanism of CD4/8 lineage commitment process, i.e., how does thymocyte stop one or the other coreceptor synthesis, is still unclear although many types of models have been presented (410).
Affiliation(s): (2) Department of Immunology, Keio University School of Medicine, Tokyo, Japan
Series: Methods in Molecular Biology  |  Volume: 134  |  Pub. Date: Dec-22-1999  |  Page Range: 63-68  |  DOI: 10.1385/1-59259-682-7:63
Subject:  Immunology
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