SpringerProtocols: Abstract: DNA-Protein In Situ Covisualization for Chromosome Analysis

DNA-Protein In Situ Covisualization for Chromosome Analysis

By: Henry H. Q. Heng², Barbara Spyropoulos³, Peter B. Moens³

Abstract

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Although fluorescent in situ hybridization (FISH) technology has been used extensively for gene mapping and genome analysis (1-8), methods that visualize the interaction of DNA and protein are required to elucidate the functional aspects of the chromosome. By displaying the physical sites of molecular reactions in the highly organized compartments of the nucleus, this methodology provides more specific information on chromatin domain, chromosome, and nuclear position than data generated by other non-in situ DNA-protein interaction assays such as gel retardation analysis.

Affiliation(s): (2) Center for Molecular Medicine and Genetics, School of Medicine, Wayne State University, Detroit, MI
(3) Department of Biology, York University, Downsview, Ontario, Canada

Book Title: In Situ Hybridization Protocols

Series: Methods in Molecular Biology | Volume: 123 | Pub. Date: Sep-01-1999 | Page Range: 15-27 | DOI: 10.1385/1-59259-677-0:15

Subject: Cell Biology

References

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1.	Lawrence, B. J. (1990) A fluorescence in situ hybridization approach for gene mapping and the study of nuclear organization, in Genome Analysis, vol I: Genetic and Physical Mapping (Davies, K. E. and Tilghman, S. M., eds.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp. 1–38.

2.	Lichter, P., Boyle, A., Cremer, T., and Ward, D. C. (1991) Analysis of genes and chromosomes by nonisotopic in situ hybridization. GATA 8, 24–35.

3.	Trask, J. B. (1991) Fluorescence in situ hybridization. TIG 7, 149–154.

4.	Spyropoulos, B. and Moens, P. B. (1994) In situ hybridization of meiotic prophase chromosomes, in Methods in Molecular Biology: In Situ Hybridization Protocols (Choo, K. H., ed.), Humana Press, Totowa, NJ, pp. 131–139.

5.	Heng, H. H. Q. and Tsui, L.-C. (1994) FISH detection on DAPI banded chromosomes, in Methods in Molecular Biology: In Situ Hybridization Protocols (Choo, K. H., ed.), Humana Press, Totowa, NJ, pp. 35–49.

6.	Heng, H. Q. H., and Tsui, L.-C. (1994) Free chromatin mapping by FISH, in Methods in Molecular Biology: In Situ Hybridization Protocols (Choo, K. H., ed.), Humana Press, Totowa, NJ, pp. 109–122.

7.	Heng H. H. Q., Tsui L.-C., Windle B. and Parra I. (1995). High-resolution FISH analysis, in Current Protocols in Human Genetics (Dracopoli, N. C., ed.), John Wiley and Sons, New York, pp. 4.5.1–4.5.26.

8.	Heng, H. H. Q., Spyropoulos, B., and Moens, P. B. (1997) FISH technology in chromosome and genome research. Bioessays 19, 75–84.

9.	Heng H. H. Q., Tsui L.-C., and Moens P. B. (1994) Organization of heterologous DNA inserts on the mouse meiotic chromosome core. Chromosoma 103, 401–407.

10.	Heng, H. H. Q., Chamberlain, J. W., Shi, X.-M., Spyropoulos, B., Tsui, L.-C., and Moens, P. B. (1996) Regulation of meiotic chromatin loop size by chromosome position. Proc. Natl. Acad. Sci. USA 93, 2795–2800.

11.	Moens, P. B., Heng, H. H. Q., Pearlman, R. E., Rosonina, E., and Spyropoulos, B. (1995) Meiotic chromosomes visualized with antibodies and DNA probes, in Kew Chromosome Conference IV (Brandham, P. E. and Bennett, M. D., eds.), Royal Botanical Gardens, Kew, pp. 375–379.

12.	Barlow, A. L. and Hulten, M. A. (1996) Combined immunocytogenetics and molecular cytogenetic analysis of meiosis I human spermatocytes. Chromosome Res. 4, 562–573.

13.	Barlow, A. L. and Hulten, M. A. (1997) Sequential immunocytogenetics, molecular cytogenetics and transmission electron microscopy of microspread meiosis I oocytes from a human fetal carrier of an unbalanced translocation. Chromosoma 106, 293–303.

14.	Moens, P. B., Heddle, J. A. M., Spyropoulos, B., and Heng, H. H. Q. (1997) Identical megabase transgenes on mouse chromosomes 3 and 4 do not promote ectopic pairing or synapsis at meiosis. Genome 40, 770–773.

15.	Moens, P., Pearlman, R., Traut, W., and Heng, H. H. Q. (1998) Chromosome cores and chromatin at meiotic prophase, in Meiosis and Gametogenesis (Handel, M., ed.), Academic, San Diego, pp. 241–262.

16.	Haaf, T. and Ward, D.C. (1994) Structural analysis of α-satellite DNA and centromeric proteins using extended chromatin and chromosomes. Hum. Mol. Genet. 3, 679–709.

17.	Hunt, P., LeMaire, R., Emburg, P, Sheean, L., and Mroz, K. (1995) Analysis of chromosome behaviour in intact mammalian oocytes: monitoring the segregation of a univalent chromosome during female meiosis. Hum. Mol. Genet. 4, 2007–2012.

18.	van den Berg, H., Vossen, J. M., van den Bergh, L., Bayer, J., and van Tol, M. J. D. (1991) Detection of Y chromosome by in situ hybridization in combination with membrane antigens by two-color immunofluorescence. Lab. Invest. 64, 623–628.

19.	Spyropoulos, B., Heng, H. H. Q., and Moens, P. B. (1997) Recycling cells in FISH and immunocytology studies. Technical Tips on Line, 25 June, T01090.

20.	Heng, H. H. Q., Squire, J., and Tsui, L.-C. (1991) Chromatin mapping—a strategy for physical characterization of the human genome by hybridization in situ. Proc. 8th Int. Cong. Human Genetics, Am J. Hum. Genet. Suppl. p. 368.

21.	Heng, H. Q. H., Squire, J., and Tsui, L.-C. (1992) High resolution mapping of mammalian genes by in situ hybridization to free chromatin. Proc. Natl. Acad. Sci. USA 89, 9509–9513.

22.	Wiegant, J., Kalle, W., Mullenders, L., Brookes, S., Hoovers, J. M. N., Dawerse, J. G., van Ommen, G. J. B., and Raap, A. K. (1992) High resolution in situ hybridization using DNA halo preparations. Hum. Mol. Genet. 1, 587–592.

23.	Heng, H. H. Q. and Tsui, L.-C. (1998) High resolution free chromatin/DNA fiber fluorescent in situ hybridization. J. Chromatogr. A 806, 219–229.

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