SpringerProtocols: Abstract: Separation of Enantiomers by Thin-Layer Chromatography: An Overview

Separation of Enantiomers by Thin-Layer Chromatography: An Overview

By: Kurt GÃ¼nther², Peter Richter², Klaus MÃ¶ller³

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The present review discusses the versatile applicability and separation mechanisms of thin-layer chromatographic enantiomeric separations. More detailed descriptions will be given for practical applications—separations of underivatized samples—on commercially available, ready-to-use plates, focusing on the thin-layer chromatographic racemate separation based on ligand exchange, which was introduced in 1983 by GÃ¼nther et al. (1) and on use of a densitometer for determination of antipode distributions at trace level. This chapter will not discuss the numerous and interesting diastereomeric separations by paper and thin-layer chromatography (TLC). We refer to the literature on amino acids (2–8), peptides, diketopiperazines (9–18), and other classes of compounds (19–31).

Affiliation(s): (2) Degussa AG, Industriepark Wolfgang GmbH, Hanau, Germany
(3) Macherey-Nagel, DÃ¼ren, Germany

Book Title: Chiral Separations: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 243 | Pub. Date: Dec-15-2003 | Page Range: 29-59 | DOI: 10.1385/1-59259-648-7:029

Subject: Biochemistry

References

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