SpringerProtocols: Abstract: Yeast Cell Synchronization

Yeast Cell Synchronization

By: Audra Day², Colette Schneider², Brandt L. Schneider²

Abstract

Full Text

Download PDF (456K)

Saccharomyces cerevisiae, the budding yeast, is widely used as a model eukaryote to study a large number of cellular processes including cell cycle regulation (1–4). Extensive genetic research in the last two decades has revealed that the basic mechanism of cell cycle control is highly conserved in all eukaryotic cells (4,6). These observations combined with powerful genetic, molecular, and biochemical tools have frequently made S. cerevisiae the experimental organism of choice for studying the mechanisms that regulate cell cycle progression (1,4,5).

Affiliation(s): (2) Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX

Book Title: Cell Cycle Checkpoint Control Protocols

Series: Methods in Molecular Biology | Volume: 241 | Pub. Date: Nov-14-2003 | Page Range: 55-76 | DOI: 10.1385/1-59259-646-0:55

Subject: Cell Biology

References

Download References

1.	Fantes, P. and Brooks, R. (1993) The Cell Cycle: A Practical Approach, IRL Press, New York.

2.	Guthrie, C, Fink, G. R., Simon, M. I., and Abelson, J. N. (eds.) (1991) Methods in Enzymology, Vol. 194, Guide to Yeast Genetics and Molecular Biology, Academic, New York.

3.	Frederick, M., Ausubel, R. B., Robert, E., et al. (eds.) (1999) Current Protocols in Molecular Biology, Wiley, New York.

4.	Murray, A. and Hunt, T. (1993) The Cell Cycle: An Introduction, W. H. Freeman, New York.

5.	Nasmyth, K. (2001) A prize for proliferation. Cell 107, 689–701.

6.	Rose, M. D., Winston, F., and Hieter, P. (1990) Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

7.	Sherman, F. (1991) Getting started with yeast, in Methods in Enzymology, Vol. 194, Guide to Yeast Genetics and Molecular Biology (Guthrie, C., Fink, G. R., Simon, M. I., and Abelson, J. N., eds.), Academic, New York, pp. 3–21.

8.	Breeden, L. L. (1997) Alpha-factor synchronization of budding yeast. Methods Enzymol. 283, 332–341.

9.	Chu, S., DeRisi, J., Eisen, M., et al. (1998) The transcriptional program of sporulation in budding yeast. Science 282, 699–705.

10.	Creanor, J. and Toyne J. (1993) Preparation of Synchronous Cultures of the Yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, IRL Press, Oxford, England.

11.	Futcher, A. B. (1993) Analysis of the Cell Cycle in S. cerevisiae, IRL Press, Oxford, England.

12.	Futcher, B. (1999) Cell cycle synchronization. Methods Cell Sci. 21, 79–86.

13.	Johnston, L. H. and Johnson, A. L. (1997) Elutriation of budding yeast. Methods Enzymol. 283, 342–350.

14.	Mitchison, J. M. (1988) Synchronous Cultures and Age Fractionation, IRL Press, Washington, DC.

15.	Walker, G. M. (1999) Synchronization of yeast cell populations. Methods Cell Sci. 21, 87–93.

16.	Cross, F. R. (1995) Starting the cell cycle: what’s the point? Curr. Opin. Cell Biol. 7, 790–797.

17.	Primig, M., Williams, R. M., Winzeler, E. A., et al. (2000) The core meiotic transcriptome in budding yeasts. Nat. Genet. 26, 415–423.

18.	Cao, L., Alani, E., and Kleckner, N. (1990) A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae. Cell 61, 1089–1101.

19.	Alani, E., Padmore, R., and Kleckner, N. (1990) Analysis of wild-type and rad50 mutants of yeast suggests an intimate relationship between meiotic chromosome synapsis and recombination. Cell 61, 419–436.

20.	Stuart, D., and Wittenberg, C. (1998) CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint. Genes Dev. 12, 2698–2710.

21.	Woldringh, C. L., Fluiter, K., and Huls, P. G. (1995) Production of senescent cells of Saccharomyces cerevisiae by centrifugal elutriation. Yeast 11, 361–369.

22.	Egilmez, N. K., Chen, J. B., and Jazwinski, S. M. (1990) Preparation and partial characterization of old yeast cells. J. Gerontol. 45, B9–17.

23.	Egilmez, N. K., Chen, J. B., and Jazwinski, S. M. (1989) Specific alterations in transcript prevalence during the yeast life span. J. Biol. Chem. 264, 14,312–14,317.

24.	Ashrafi, K., Sinclair, D., Gordon, J. I., and Guarente, L. (1999) Passage through stationary phase advances replicative aging in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA. 96, 9100–9105.

25.	Haase, S. B. and Lew, D. J. (1997) Flow cytometric analysis of DNA content in budding yeast. Methods Enzymol. 283, 322–332.

26.	Spellman, P. T., Sherlock, G., Zhang, M. Q., et al. (1998) Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol. Biol. Cell. 9, 3273–3297.

27.	Pringle, J. R., Adams, A. E., Drubin, D. G., and Haarer, B. K. (1991) Immunofluorescence methods for yeast. Methods Enzymol. 194, 565–602.

28.	Hartwell, L. H., and Unger, M. W. (1977) Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division. J. Cell. Biol. 75, 422–435.

29.	Johnston, G. C., Pringle, J. R., and Hartwell, L. H. (1977) Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp. Cell Res. 105, 79–98.

Comments (Loading...)

Post comment/View all comments