SpringerProtocols: Abstract: Methods for Detecting Cells in S Phase

Methods for Detecting Cells in S Phase

By: Wei-Hsin Sun², Melvin L. DePamphilis³

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S phase is that period of time in the cell-division cycle during which nuclear chromosomal deoxyribonucleic acid (DNA) is replicated (1,2). The time required for S phase depends on the size of the genome, the organism, and its developmental state. DNA replication requires only 15 to 20 min in budding yeast, but 6 to 7 h in mammalian cells. In organisms, such as frogs, fish, echinoderms, and flies that undergo rapid cell cleavage events at the beginning of their development, S phase takes only a few minutes during these initial cell cleavage events, but it takes several hours in late-stage embryos, adult animals, or cells cultured in vitro.

Affiliation(s): (2) National Institute of Mental Health, National Institutes of Health, Bethesda, MD
(3) National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD

Book Title: Cell Cycle Checkpoint Control Protocols

Series: Methods in Molecular Biology | Volume: 241 | Pub. Date: Nov-14-2003 | Page Range: 37-53 | DOI: 10.1385/1-59259-646-0:37

Subject: Cell Biology

References

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