Springer Protocols: Abstract: Preparation and Solubilization of Body Fluids for 2-D

Preparation and Solubilization of Body Fluids for 2-D

By: Jean-Charles Sanchez², Denis F. Hochstrasser³

Abstract

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Cells, tissues, and organs require energy to function normally. Some of them are metabolic providers, and some others are users. Body fluids act as transporters and distributors of metabolic “fuels” between cells and maintain relatively constant conditions through homeostasis. The ratio of solute to water in all body fluids is maintained within a very narrow range. The concentrations of body fluid metabolites are thus extremely well regulated. Many diseases or environment disturbances lead to changes in the expression of body fluid proteins. Information on these changes is often of diagnostic, prognostic, or therapeutic importance.

Affiliation(s): (2) Clinical Chemistry Laboratory, Geneva University Hospital, Geneva, Switzerland
(3) Clinical Chemistry Laboratory, Medical Biochemistry Department, Geneva University Hospital, Geneva, Switzerland

Book Title: 2-D Proteome Analysis Protocols

Series: Methods in Molecular Biology | Volume: 112 | Pub. Date: Sep-24-1998 | Page Range: 87-93 | DOI: 10.1385/1-59259-584-7:87

Subject: Biochemistry

References

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1.	Tracy, R. P. and Young, D. S. (1984) Clinical applications of two-dimensional gel electrophoresis, in Two-Dimensional Gel Electrophoresis of Proteins (Celis, J. and Bravo, R., eds.), Academic, Orlando, FL, pp. 193–240.

2.	Hochstrasser, D. F. and Tissot, J.-D. (1993) Clinical application of high-resolution 2-D PAGE, in Advances in Electrophoresis, vol. 6 (Chrambach, A., Dunn, M. J., and Radola, B. J., eds.), VCH, Weinheim, pp. 270–375.

3.	Giometti, C. S. and Anderson, N. G. (1980) Two-dimensional electrophoresis of human saliva, in Electrophoresis, vol. 79 (Radola, B., ed.), de Gruyter, New York, pp. 395–404.

4.	Argiles, A. (1987) Amyloid deposit in hemodialysis patients: immunochemical analysis. Nephrologie 8, 51–54.

5.	Janssen, P. T. and Van Bijsterveld, Q. P. (1981) Comparison of electrophoretic techniques for the analysis of human tear fluid pattern. Clin. Chim. Acta 114, 207.

6.	Scheele, G. A. (1975) Two-dimensional gel analysis of soluble proteins. J. Biol. Chem. 250, 5375–5385.

7.	Cassara, G., Gianazza, E., Righetti, P. G., Poma, S., Vicentini, L., and Scortecci, V. (1980) Isoelectric focusing studies on human pancreatic secretion. J. Chromatogr. 221, 279.

8.	Jones, M. I., Spragg, S. P., and Webb, T. (1981) Detection of proteins in human amniotic fluid using two-dimensional gel electrophoresis. Biol. Neonate 39, 171–177.

9.	Toussi, A., Paquet, N., Huber, O., Frutiger, S., Tissot, J. D., Hughes, G. J., et al. (1992) Polypeptide marker and disease patterns found while mapping proteins in ascitis. J. Chromatogr. 582, 87–92.

10.	Dermer, G. B. (1982) Enhancement techniques for detecting trace and fluid-specific components in two-dimensional electrophoresis patterns. Clin. Chem. 28, 881–887.

11.	Brunet, J.-F., Berger, F., Gustin, T., Laine, M., and Benabid, H. L. (1993) Characterization of normal brain-reactive antibodies in glioma cyst fluids. J. Neuroimmunol. 47, 63–72.

12.	Marshall, T. and Williams, K. M. (1984) Analysis of human sweat proteins by two-dimensional electrophoresis and ultrasensitive silver staining. Anal. Biochem. 139, 506–509.

13.	Anderson, N. G., Powers, M. T., and Tollaksen, S. L. (1982) Proteins of human Milk. I. Identification of major components. Clin. Chem. 28, 1045–1050.

14.	Edwards, J. J., Tollaksen, S. L., and Anderson, N. G. (1981) Proteins of human semen. I. Two-dimensional mapping of human seminal fluid. Clin. Chem. 27, 1335–1340.

15.	Anderson, N. L. and Anderson, N. G. (1977) High resolution two-dimensional electrophoresis of human plasma proteins. Proc. Natl. Acad. Sci. USA 74, 5421–5425.

16.	Golaz, O, Hughes, G. J., Frutiger, S., Paquet, N., Bairoch, A., Pasquali, C., et al. (1993) Plasma and red blood cell protein maps: update 1993. Electrophoresis 14, 1223–1231.

17.	Switzer, R. C., Merril, C. R., and Shifrin, S. A. (1979) A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal. Biochem. 98, 231–237.

18.	Goldman, D., Merril, C. R., and Ebert, M. H. (1980) Two-dimensional gel electrophoresis of cerebrospinal fluid proteins. Clin. Chem. 26, 1317–1322.

19.	Anderson, N. G., Anderson, N. L., and Tollaksen, S. L. (1979) Proteins of human urine. I. Concentration and analysis by two-dimensional electrophoresis. Clin. Chem. 25, 1199–1210.

20.	Edwards, J. J., Tollaksen, S. L., and Anderson, N. G. (1982) Proteins of human urine. III. Identification and two-dimensional electrophoretic map positions of some major urinary proteins. Clin. Chem. 28, 941–948.

21.	Pitt-Rivers, R. and Impiombato, F. S. A. (1968) The binding of sodium dodecyl sulphate to various proteins. Biochem. J. 109, 825.

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