Springer Protocols: Abstract: Running Preparative Carrier Ampholyte and Immobilized pH Gradient IEF Gels for 2-D

Running Preparative Carrier Ampholyte and Immobilized pH Gradient IEF Gels for 2-D

By: Neil M. Matsui², Diana M. Smith-Beckerman³, Jenny Fichmann⁴, Lois B. Epstein⁵

Abstract

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2-D gel electrophoresis is one of the most effective techniques for high-resolution separation of complex protein mixtures. Recent developments in the field of protein mass spectrometry allow the rapid and highly sensitive identification of proteins, and thus amplify the power of preparative 2-D gel electrophoresis.

Affiliation(s): (2) Department of Hematology, SFGH, San Francisco, CA
(3) Center for Biomedical Science, San Francisco State University, San Francisco, CA
(4) Amersham Pharmacia Biotech, San Francisco, CA
(5) Cancer Research Institute and the Department of Pediatrics, University of California, San Francisco, CA

Book Title: 2-D Proteome Analysis Protocols

Series: Methods in Molecular Biology | Volume: 112 | Pub. Date: Sep-24-1998 | Page Range: 211-219 | DOI: 10.1385/1-59259-584-7:211

Subject: Biochemistry

References

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