Preparation of Escherichia coli Samples for 2-D Gel Analysis
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Escherichia coli has been studied for many years by two-dimensional polyacrylamide gel electrophoresis (2-D gels) (1,2). This method has provided much information about the physiology of E. coli, particularly related to how the levels or synthesis rates of large numbers of proteins varied under different conditions.
Affiliation(s): (2) Biotechnology Laboratory, Parke-Davis Pharmaceutical Research, Ann Arbor, MI
(3) Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, MI
(3) Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, MI
Book Title: 2-D Proteome Analysis Protocols
Series: Methods in Molecular Biology | Volume: 112 | Pub. Date: Sep-24-1998 | Page Range: 21-29 | DOI: 10.1385/1-59259-584-7:21
Subject: Biochemistry
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