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Immature Inflorescence Culture of Cereals: A Highly Responsive System for Regeneration and Transformation
Abstract
Success in obtaining transgenic cereals depends largely on efficient plant regeneration protocols. It is not trivial to establish regenerable tissue cultures in cereals, since only immature tissues of a limited age range can be induced to regenerate efficiently. Generally, the tissue-culture factors that determine the success of regeneration and transformation in cereals are: genotype, choice of explant, physiological state of the explants/donor plants, culture conditions, and medium composition.
Affiliation(s): (2) Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, Hertfordshire, UK
Series: Methods in Molecular Biology  |  Volume: 111  |  Pub. Date: Feb-15-1999  |  Page Range: 71-81  |  DOI: 10.1385/1-59259-583-9:71
Subject:  Plant Sciences
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