SpringerProtocols: Abstract: Callus Initiation, Maintenance, and Shoot Induction in Rice

Callus Initiation, Maintenance, and Shoot Induction in Rice

By: Nigel W. Blackhall², Joan P. Jotham², Kasimalal Azhakanandam², J. Brian Power², Kenneth C. Lowe², Edward C. Cocking², Michael R. Davey²

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Embryogenic suspension cultures provide the most widely employed source of totipotent cells for protoplast isolation in rice (Oryza sativa L.), since mesophyll-derived protoplasts of this important cereal rarely undergo sustained mitotic division leading to the production of tissues capable of plant regeneration. Cells from embryogenic suspensions provide an alternative to immature zygotic embryos for biolistic-mediated production of fertile transgenic rice plants (1) and are also amenable to transformation procedures employing agrobacteria (2). Currently, protocols are available for regenerating fertile plants from cell suspension-derived protoplasts of the three major subgroups of rice varieties, namely japonica (3), javanica (4) and indica (5) rices.

Affiliation(s): (2) Department of Life Sciences, University of Nottingham, University Park, Nottingham, UK

Book Title: Plant Cell Culture Protocols

Series: Methods in Molecular Biology | Volume: 111 | Pub. Date: Feb-15-1999 | Page Range: 19-29 | DOI: 10.1385/1-59259-583-9:19

Subject: Plant Sciences

References

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