SpringerProtocols: Abstract: 3. Mapping of the Hotspots of Recombination in Human DNA Cloned as Yeast Artificial Chromosomes

3. Mapping of the Hotspots of Recombination in Human DNA Cloned as Yeast Artificial Chromosomes

By: Grzegorz Ira², Jacek Król², Jan Filipski²

Abstract

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Whole genome sequencing of several species, including our own, constitutes a good starting point for the study of various functions of the chromosome on a whole genome scale. Mapping of meiotic recombination hotspots (the sites in which recombination occurs more frequently than at an average site) in the genomes of higher organisms is one of the tasks that can now be undertaken. Hotspot mapping, in the case of the human genome, could be important for understanding the origin of some genetic diseases and cancers and might shed light on the mechanisms of evolution of our species at the molecular level.

Affiliation(s): (2) Laboratoire de Biochimie de la Chromatine, Institute Jacques Monod, Universitiés Paris 6 and Paris 7, Paris, France

Book Title: Mammalian Artificial Chromosomes: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 240 | Pub. Date: Nov-30-2003 | Page Range: 35-52 | DOI: 10.1385/1-59259-434-4:35

Subject: Genetics/Genomics

References

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