SpringerProtocols: Abstract: 12. Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice: Pick-and-Inject Technique

12. Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice: Pick-and-Inject Technique

By: Diane P. Monteith², Josephine D. Leung², Anita H. Borowski³, Deborah O. Co², Tünde Praznovszky⁴, Frank R. Jirik⁵, Gyula Hadlaczky^{2 5}, Carl F. Perez⁵

Abstract

Full Text

Download PDF (146K)

Mammalian artificial chromosomes (MACs) are appealing vectors for transgenesis and gene therapy applications. Their principal benefits include large DNA-carrying capacity and the ability to replicate in synchrony with the host genome. In addition, introducing genes into artificial chromosomes eliminates the disadvantages of integrating exogenous DNA into the host genome—variegated gene expression and insertional mutagenesis.

Affiliation(s): (2) Chromos Molecular Systems Inc., Burnaby, British Columbia, Canada
(3) Abgenix Biopharma Inc., Vancouver, British Columbia, Canada
(4) Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary
(5) Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada

Book Title: Mammalian Artificial Chromosomes: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 240 | Pub. Date: Nov-30-2003 | Page Range: 227-242 | DOI: 10.1385/1-59259-434-4:227

Subject: Genetics/Genomics

References

Download References

1.	Harrington, J. J., Van Bokkelen, G., Mays, R. W., Gustashaw, K., and Willard, H. F. (1997) Formation of de novo centromeres and construction of first-generation human artificial microchromosomes. Nat. Genet. 15, 345–355.

2.	Warburton, P. E. and Cooke, H. J. (1997) Hamster chromosomes containing amplified human alphα-satellite DNA show delayed sister chromatid separation in the absence of de novo kinetochore formation. Chromosoma 106, 145–159.

3.	Ikeno, M., Grimes, B., Okazaki, T., Nakano, M., Saitoh, K., Hoshino, H., et al. (1998) Construction of YAC-based mammalian artificial chromosomes. Nat. Biotech. 16, 431–439.

4.	Henning, K. A., Novotny, E. A., Compton, S. T., Guan, X. Y., Liu, P. P., and Ashlock, M. A. (1999) Human artificial chromosomes generated by modification of a yeast artificial chromosome containing both human alpha satellite and single-copy sequences. Proc. Natl. Acad. Sci. USA 96, 592–597.

5.	Ebersole, T. A., Ross, A., Clark, E., McGill, N., Schindelhauer, D., Cooke, H., and Grimes, B. (2000) Mammalian artificial chromosome formation from circular alphoid input DNA does not require telomere repeats. Hum. Mol. Genet. 9, 1623–1631.

6.	Carine, K., Solus, J., Waltzer, E., Manch-Citron, J., Hamkalo, B., and Scheffler, I. (1986) Chinese hamster cells with a minichromosome containing the centromere region of human chromosome 1. Somat. Cell Mol. Genet. 12, 479–491.

7.	Farr, C., Fantes, J., Goodfellow, P., and Cooke, H. (1991) Functional reintroduction of human telomeres into mammalian cells. Proc. Natl. Acad. Sci. USA 88, 7006–7010.

8.	Farr, C. J., Stevanic, M., Thomson, E. J., Goodfellow, P. N., and Cooke, H. J. (1992) Telomere-associated chromosome fragmentation: applications in genome manipulation and analysis. Nat. Genet. 2, 275–282.

9.	Barnett, M. A., Buckle, V. J., Evans, E. P., Porter, A. C., Rout, D., Smith, A. G., and Brown, W. R. (1993) Telomere directed fragmentation of mammalian chromosomes. Nucleic Acids Res. 21, 27–36.

10.	Farr, C. J., Bayne, R. A., Kipling, D., Mills, W., Critcher, R., and Cooke, H. J. (1995) Generation of a human X-derived minichromosome using telomere-associated chromosome fragmentation. EMBO J. 14, 5444–5454.

11.	Heller, R., Brown, K. E., Burgtorf, C., and Brown, W. R. (1996) Minichromosomes derived from the human Y chromosome by telomere directed chromosome breakage. Proc. Natl. Acad. Sci. USA 93, 7125–7130.

12.	Shen, M. H., Yang, J., Loupart, M. L., Smith, A., and Brown W (1997) Human mini-chromosomes in mouse embryonal stem cells. Hum. Mol. Genet. 6, 1375–1382.

13.	Au, H. C., Mascarello, J. T., and Scheffler, I. E. (1999) Targeted integration of a dominant neo® marker into a 2-to 30-Mb human minichromosome and transfer between cells. Cytogenet. Cell Genet. 86, 194–203.

14.	Mills, W., Critcher, R., Lee, C., and Farr, C. J. (1999) Generation of an approximately 2.4 Mb human X centromere-based minichromosome by targeted telomere-associated chromosome fragmentation in DT40. Hum. Mol. Genet. 8, 751–761.

15.	Shen, M. H., Mee, P. J., Nichols, J., Yang, J., Brook, F., Gardner, R. L., et al. (2000) A structurally defined mini-chromosome vector for the mouse germ line. Curr. Biol. 10, 31–34.

16.	Voet, T., Vermeesch, J., Carens, A., Durr, J., Labaere, C., Duhamel, H., et al. (2001) Efficient male and female germline transmission of a human chromosomal vector in mice. Genome Res. 11, 124–136.

17.	Krysan, P. J., Haase, S. B., and Calos, M. P. (1989) Isolation of human sequences that replicate autonomously in human cells. Mol. Cell. Biol. 9, 1026–1033.

18.	Sun, T. Q., Fernstermacher, D. A., and Vos, J. M. (1994) Human artificial episomal chromosomes for cloning large DNA fragments in human cells. Nat. Genet. 8, 33–41.

19.	Sun, T. Q., Livanos, E., and Vos, J. M. (1996) Engineering of a miniherpesvirus as a general strategy to transduce up to 180 kb of functional self-replicating human mini-chromosomes. Gene Ther. 3, 1081–1088.

20.	Calos, M. P. (1996) The potential of extrachromosomal replicating vectors for gene therapy. Trends Genet. 12, 463–466.

21.	Simpson, K., McGuigan, A., and Huxley, C. (1996) Stable episomal maintenance of yeast artificial chromosomes in human cells. Mol. Cell. Biol. 16, 5117–5126.

22.	Kelleher, Z. T., Fu, H., Livanos, E., Wendelburg, B., Gulino, S., and Vos, J. M. (1998) Epstein-Barr-based episomal chromosomes shuttle 100 Kb of self-replicating circular human DNA in mouse cells. Nat. Biotechnol. 16, 762–768.

23.	Hadlaczky, G., Praznovszky, T., Cserpán, I., Keresö, J., Péterfy, M., Kelemen, I., et al. (1991) Centromere formation in mouse cells cotransformed with human DNA and a dominant marker gene. Proc. Natl. Acad. Sci. USA 88, 8106–8110.

24.	Praznovszky, T., Keresö, J., Tubak, V., Cserpán, I., Fátyol, K., and Hadlaczky, G. (1991) De novo chromosome formation in rodent cells. Proc. Natl. Acad. Sci. USA 88, 11042–11046.

25.	Keresö, J., Praznovszky, T., Cserpán, I., Fodor, K., Katona, R., Csonka, E., et al. (1996) De novo chromosome formations by largescale amplification of the centromeric region of mouse chromosomes. Chrom. Res. 4, 226–239.

26.	Holló, G., Keresö, J., Praznovszky, T., Cserpán, I., Fodor, K., Katona, R., et al. (1996) Evidence for a megareplicon covering megabases of centromeric chromosome segments. Chrom. Res. 4, 240–247.

27.	Csonka, E., Cserpán, I., Fodor, K., Holló, G., Katona, R., Keresö, J., et al. (2000) Novel generation of human satellite DNA-based artificial chromosomes in mammalian cells. J. Cell Sci. 113, 3207–3216.

28.	de Jong, G., Telenius, A. H., Telenius, H., Perez, C. F., Drayer, J. I., and Hadlaczky, G. (1999) Mammalian artificial chromosome pilot production facility: large-scale isolation of functional satellite DNA-based artificial chromosomes. Cytometry 35, 129–133.

29.	Telenius, H., Szeles, A., Keresö, J., Csonka, E., Praznovszky, T., Imreh, S., et al. (1999) Stability of a functional murine satellite DNA-based artificial chromosome across mammalian species. Chrom. Res. 7, 3–7.

30.	Co, D. O., Borowski, A. H., Leung, J. D., van der Kaa, J., Hengst, S., Platenburg, G. J., et al. (2000) Generation of transgenic mice and germline transmission of a mammalian artificial chromosome introduced into embryos by pronuclear microinjection. Chrom. Res. 8, 183–191.

31.	Tomizuka, K., Yoshida, H., Uejima, H., Kugoh, H., Sato, K., Ohguma, A., et al. (1997) Functional expression and germline transmission of a human chromosome fragment in chimaeric mice. Nat. Genet. 16, 133–143.

32.	Hernandez, D., Mee, P. J., Martin, J. E., Tybulewicz, V. L., and Fisher, E. M. (1999) Transchromosomal mouse embryonic stem cell lines and chimeric mice that contain freely segregating segments of human chromosome 21. Hum. Mol. Genet. 8, 923–933.

33.	Tomizuka, K., Shinohara, T., Yoshida, H., Uejima, H., Ohguma, A., Tanaka, S., et al. (2000) Double trans-chromosomic mice: maintenance of two individual human chromosome fragments containing Ig heavy and {k} loci and expression of fully human antibodies. Proc. Natl. Acad. Sci. USA 97, 722–727.

34.	Murphy, D. and Carter, D. A. (eds.) Transgenesis Techniques. Humana, Totowa, NJ, 1993.

35.	Carter, N. P. Bivariate chromosome analysis using a commercial flow cytometer, in Chromosome Analysis Protocols (Gosden, J. R., ed.), Humana, Totowa, NJ, 1994, pp. 187–204.

36.	Fantes, J. A., Green, D. K., and Sharkey, A. Chromosome sorting by flow cytometry, in Chromosome Analysis Protocols (Gosden, J. R., ed.), Humana, Totowa, NJ, 1994, pp. 205–219.

37.	Hogan, B., Beddington, R., Costantini, F., and Lacy, E. (eds.) Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, NY, 1994.

38.	Hem, A., Smith, A. J., and Solberg, P. (1998) Saphenous vein puncture for blood sampling of the mouse, rat, hamster, gerbil, guinea pig, ferret, and mink. Lab. Anim. 32, 364–368.

39.	Dracopoli, N. C., Haines, J. L., Korf, B. R., Moir, D. T., Morton, C. C., Seidman, C. E., et al. Clinical cytogenetics, in Current Protocols in Human Genetics. John Wiley and Sons, NY, 1994.

40.	Pinkel, D., Straume, T., and Gray, J. W. (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc. Natl. Acad. Sci. USA 83, 2937–2938.

Comments (Loading...)

Post comment/View all comments