SpringerProtocols: Abstract: Multiplex PCR for the Detection of the Factor V Leiden and Prothrombin 20210A Mutations

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Multiplex PCR for the Detection of the Factor V Leiden and Prothrombin 20210A Mutations

By: Anne M. Sproul², Elizabeth A. Chalmers²

Abstract

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An extensive published literature now exists on the role of heritable thrombophilia in adult thromboembolic disease (1). Activated protein C resistance (APCR) secondary to the factor V (FV) Leiden mutation is a relatively common genetic defect, occurring in 2–15% of Caucasian populations and 20–50% of adults with a first episode of venous thromboembolism (2). Similarly, the PT20210A mutation, which results in increased levels of prothrombin, has a prevalence of approx 2% in the normal population and 6% in unselected adults with venous thrombosis (3).

Affiliation(s): (2) Department of Haematology, Royal Hospital for Sick Children, Glasgow, UK

Book Title: Pediatric Hematology: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 91 | Pub. Date: Sep-29-2003 | Page Range: 79-87 | DOI: 10.1385/1-59259-433-6:79

Subject: Molecular Medicine

References

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