SpringerProtocols: Abstract: 13. Analysis of the Coupling of G12/13 to G Protein-Coupled Receptors Using a Luciferase Reporter Assay

13. Analysis of the Coupling of G12/13 to G Protein-Coupled Receptors Using a Luciferase Reporter Assay

By: Bo Liu², Dianqing Wu²

Abstract

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G protein-coupled receptors (GPCRs) transduce signals from cell surface to intracellular effectors through various G proteins. In this chapter, a luciferase reporter gene assay system is described for the study of the coupling of GPCRs to Gα_12/13. This assay system, in which ligand-stimulated production of the reporter luciferase under the control of a modified serum-responsive element (SRE) in a cell line derived from mice lacking Gα_q/11 that are transfected with a GPCR of interest, allows for easy, fast, and valid determination of the coupling of the GPCR to Gα_12/13.

Affiliation(s): (2) Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT

Book Title: G Protein Signaling: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 237 | Pub. Date: Sep-15-2003 | Page Range: 145-149 | DOI: 10.1385/1-59259-430-1:145

Subject: Protein Science

Key Words: G12 - G13 - G proteins - GPCR - SRE - luciferase

References

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