9. Isolation of Plasmids from E. coli by Boiling Lysis
The boiling lysis procedure (
1) is quick to perform and, therefore, especially suitable for screening large numbers of small-volume
Escherichia coli cultures. It is described with different adaptations in a variety of protocol books (
2,
3). The quality of the isolated plasmid DNA is lower than that from an alkaline lysis miniprep, but it is sufficient for restriction
analysis.
Affiliation(s): (3) Department of Microbiology, Weill Medical College of Cornell University, New York, NY Series: Methods in Molecular Biology | Volume: 235 | Pub. Date: Jul-03-2003 | Page Range: 79-82 | DOI: 10.1385/1-59259-409-3:79
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