SpringerProtocols: Abstract: Detection and Selection of Lentiviral Vector-Transduced Cells

Detection and Selection of Lentiviral Vector-Transduced Cells

By: Yan Cui², Lung-Ji Chang^{3 4}

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Lentiviruses are members of a subgroup of enveloped Retroviridae. Lentivirus-based gene delivery vectors have gained popularity in gene therapy field because of their notable potentials in delivering and integrating transgenes into both mitotic active and inactive cells in vitro and in vivo, a characteristic that overcomes many of the barriers of therapeutic gene therapy application. In the past few years, different lentiviral vector systems have been developed based on primate and nonprimate lentiviruses, including human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus (SIV), equine infectious anemia virus (EIAV), and feline immunodeficiency virus (FIV) (see refs. 1–4). However, HIV-1-based lentiviral vector remains the best-studied and well-developed lentiviral vector system that has shown high transduction efficiencies targeting many human and experimental animal cell types, including stem cells (embryonic and adult) (5–10), terminally differentiated somatic cells (i.e., neurons) (7,11,12), muscles (7,13,14), skin (15), liver (13,16,17), islet (18,19), lung epithelium (20,21), retina (22,23), primary T lymphocytes (24,25), and fetal tissues (21,26) (Table 1).

Affiliation(s): (2) Gene Therapy Program, Stanley S. Scott Cancer Center, Department of Medicine, Louisiana State University Health Sciences Center, LA
(3) Department of Molecular Genetics and Microbiology, Powell Gene Therapy Center, University of Florida, Gainesville, FL
(4) McKnight Brain Institute, University of Florida, Gainesville, FL

Book Title: Lentivirus Gene Engineering Protocols

Series: Methods in Molecular Biology | Volume: 229 | Pub. Date: Apr-30-2003 | Page Range: 69-85 | DOI: 10.1385/1-59259-393-3:69

Subject: Genetics/Genomics

References

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