SpringerProtocols: Abstract: Opioid Receptor Oligomerization: Detection and Functional Characterization of Interacting Receptors

Opioid Receptor Oligomerization: Detection and Functional Characterization of Interacting Receptors

By: Ivone Gomes², Lakshmi A. Devi², Julija Filipovska³

Abstract

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Opioid receptors are members of the G-protein coupled receptor (GPCR) family characterized by the presence of seven transmembrane domains. These receptors are classified as mu (μ), delta (δ), and kappa (κ). Activation of these receptors leads to uncoupling of the inhibitory G-proteins (G_i) followed by the activation of multiple signal transduction pathways including the inhibition of adenylyl cyclase, modulation of inwardly rectifying K⁺ channels or voltage-dependent calcium channels and regulation of mitogen-activated protein (MAP) kinase activity (1–4).

Affiliation(s): (2) Department of Pharmacology and Biological Chemistry, Mount Sinai, New York, NY
(3) Department of Biochemistry and Molecular Biology, University of Barcelona, Barcelona, Spain

Book Title: Opioid Research: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 84 | Pub. Date: Apr-08-2003 | Page Range: 157-183 | DOI: 10.1385/1-59259-379-8:157

Subject: Pharmacology/Toxicology

References

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1.	Herz, A. (1993) Opioids, vol. 1, Springer-Verlag, Berlin, Germany.

2.	Jordan, B. A. and Devi, L. A. (1998) Molecular mechanisms of opioid receptor signal transduction. Br. J. Anaesth. 81, 12–19.

3.	Li, L.Y. and Chang, K. J. (1996) The stimulatory effects of opioids on mitogen activated protein kinase in Chinese hamster ovary cells transfected to express muopioid receptors. Mol. Pharmacol. 50, 599–602.

4.	Sarne, Y., Fields, A., Keren, O., and Gafni, M. (1996) Stimulatory effects of opioids on transmitter release and possible cellular mechanisms: overview and original results. Neurochem. Res. 21, 1353–1361.

5.	Gomes, I., Jordan, B. A., Rios, C., Trapaidze, N., and Devi, L. A. (2001) G-protein coupled receptor dimerization: implications in modulating receptor function. J. Mol. Med. 79, 226–242.

6.	Rios, C. D., Jordan, B. A., Gomes, I., and Devi L. A. (2001) G-protein-coupled receptor dimerization: modulation of receptor function. Pharmacol. Ther. 92, 71–87.

7.	Cvejic, S. and Devi, L. A. (1997). Dimerization of the delta opioid receptor: implication for a role in receptor internalization. J. Biol. Chem. 272, 26,959–26,964.

8.	Jordan, B. A. and Devi, L. A. (1999) G-protein-coupled receptor heterodimerization modulates receptor function. Nature 399, 697–700.

9.	Gomes, I., Jordan, B.A., Gupta, A., Trapaidze, N., Nagy, V., and Devi L. A. (2000) Heterodimerization of mu and delta opioid receptors: A role in opiate synergy. J. Neurosci. 20, RC110, 1–5.

10.	Jordan, B. A., Trapaidze, N., Gomes, I., Nivarthi, R., and Devi, L. A. (2001) Oligomerization of opioid receptors with beta 2-adrenergic receptors: a role in trafficking and mitogen-activated protein kinase activation. Proc. Natl. Acad. Sci. USA 98, 343–348.

11.	Angers, S., Salahpour, A., and Bouvier, M. (2001) Biochemical and biophysical demonstration of GPCR oligomerization in mammalian cells. Life Sci. 68, 2243–2250.

12.	Trapaidze, N., Gomes, I., Cvejic, S., Bansinath, M., and Devi, L. A. (2000) Opioid receptor endocytosis and activation of MAP kinase pathway. Mol. Brain Res. 76, 220–228.

13.	Trapaidze, N., Gomes, I., Bansinath, M., and Devi, L. A. (2000) Recycling and resensitization of delta opioid receptors. DNA Cell Biol. 19, 195–204.

14.	Gomes, I., Trapaidze, N., Turndorf, H., Devi, L. A., and Bansinath, M. (2000) Acute ethanol treatment modulates delta opioid receptors in N18TG2 cells. Anesthesiology 92, 1789–1798.

15.	Kroeger, K. M., Hanyaloglu, A. C., Seeber, R. M., Miles, L. E., and Eidne, K. A. (2001) Constitutive and agonist-dependent homo-oligomerization of the thyrotropin-releasing hormone receptor. Detection in living cells using bioluminescence resonance energy transfer. J. Biol. Chem. 276, 12,736–12,743.

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