SpringerProtocols: Abstract: 13. Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates

13. Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates

By: Eileen L. Whiteman², Morris J. Birnbaum^{3 4}

Abstract

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This chapter describes techniques to successfully detect tyrosine phosphorylation of the insulin receptor and insulin receptor substrate proteins. These assays demonstrate whether the insulin signaling pathway is activated at its earliest stages.

Affiliation(s): (2) Howard Hughes Medical Institute, Cell and Molecular Biology Graduate Group, University of Pennsylvania School of Medicine, Philadelphia, PA
(3) Howard Hughes Medical Institute, Cox Institute, Philadelphia, PA
(4) University of Pennsylvania School of Medicine, Philadelphia, PA

Book Title: Diabetes Mellitus: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 83 | Pub. Date: Feb-04-2003 | Page Range: 119-126 | DOI: 10.1385/1-59259-377-1:119

Subject: Molecular Medicine

References

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