SpringerProtocols: Abstract: Isolation of Polysomal RNA for Microarray Analysis

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Isolation of Polysomal RNA for Microarray Analysis

By: Yoav Arava³

Abstract

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DNA microarrays have been used extensively in recent years to study mRNA expression profiles of different cell types under various growth conditions. These steady-state mRNA profiles provide a wealth of information about cellular functions and responses. However, they do not necessarily reflect the ultimate gene expression profile of a cell since the step of translation might lead to discrepancies between the mRNA profile and the profile of the actual functioning unit in the cell, the protein (e.g., 1,2).

Affiliation(s): (3) Department of Biochemistry, Stanford University School of Medicine, Palo Alto, CA

Book Title: Functional Genomics: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 224 | Pub. Date: Mar-13-2003 | Page Range: 79-87 | DOI: 10.1385/1-59259-364-X:79

Subject: Genetics/Genomics

References

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