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Isolation, Culture, and Gene Transfer of Adult Canine Cardiac Myocytes
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The ability to isolate individual canine cardiac myocytes that maintain normal rod-shaped morphology and contractility with
stimulation is integral to the study of cardiac myocyte function and response to intervention in vitro. The literature is
replete with the use of isolated murine and rat myocytes for the investigation of cardiac physiology. Methods include morselization
or perfusion of the myocardium with various enzymatic solutions to disperse cells. Similar methods have been translated to
larger species with variable yield and viability.
Affiliation(s): (3) Department of Surgery, University of Michigan Health System, Ann Arbor, MI
(4) Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI
(5) Department of Physiology, University of Michigan, Ann Arbor, MI
(6) Department of Internal Medicine, University of Michigan, Ann Arbor, MI
(7) Center for Integrative Genomics, University of Michigan, Ann Arbor, MI
(4) Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI
(5) Department of Physiology, University of Michigan, Ann Arbor, MI
(6) Department of Internal Medicine, University of Michigan, Ann Arbor, MI
(7) Center for Integrative Genomics, University of Michigan, Ann Arbor, MI
Series: Methods in Molecular Biology | Volume: 219 | Pub. Date: Dec-19-2002 | Page Range: 145-157 | DOI: 10.1385/1-59259-350-X:145
Subject: Genetics/Genomics
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