SpringerProtocols: Abstract: Gene Gun-Based In Vivo Gene Transfer: Application to DNA Vaccination

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Gene Gun-Based In Vivo Gene Transfer: Application to DNA Vaccination

By: Tohru Sakai², Kunisuke Himeno³

Abstract

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The introduction of genetic material into tissue of interest remains a ratelimiting step for molecular investigations in many fields. Many different methods have been developed for the delivery of a gene in vivo, such as virus-, cationic liposome-, and particle-mediated gene transfer or direct injection of DNA.

Affiliation(s): (2) Department of Nutrition, University of Tokushima School of Medicine, Tokushima, Japan
(3) Department of Parasitology and Immunology, Kyushu University Graduate School of Medicine, Fukuoka, Japan

Book Title: Cytokines and Colony Stimulating Factors: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 215 | Pub. Date: Nov-08-2002 | Page Range: 181-191 | DOI: 10.1385/1-59259-345-3:181

Subject: Immunology

References

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3.	Cheng, L., Ziegelholder, R. P., and Yang, N.-S. (1993) In vivo promotor activity and transgene expression in mammalian somatic tissues evaluated by using particle bombardment. Proc. Natl. Acad. Sci. USA 90, 4455–4459.

4.	Donnelly, J. J., Ulmer, J. B., Shiver, J. W., and Liu, M. A. (1997) DNA vaccines. Annu. Rev. Immunol. 15, 617–648.

5.	Rakhmilevick, A. L., Turner, J., Ford, M. J., McCabe, D., Sun, W. H., Sondel, P. M., et al. (1996) Gene gun-mediated skin transfection with interleukin 12 gene results in regression of established primary and metastatic tumors. Proc. Natl. Acad.Sci. USA 93, 6291–6296.

6.	Sakai, T., Horii, T., Hisaeda, H., Zhang, M., Ishii, K., Nakano, Y., et al. (1999) DNA immunization with Plasmodium serine repeat antigen: regulation of humoral immune response by coinoculation of cytokine expression plasmid. Parasitol. Int. 48, 27–33. <Occurrence Type="DOI"><Handle>10.1016/S1383-5769(98)00036-1</Handle></Occurrence>

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