SpringerProtocols: Abstract: Construction of Multiply Disabled Herpes Simplex Viral Vectors for Gene Delivery to the Nervous System

Construction of Multiply Disabled Herpes Simplex Viral Vectors for Gene Delivery to the Nervous System

By: Caroline E. Lilley², Robert S. Coffin^{3 4}

Abstract

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This chapter aims to describe the main procedures involved in constructing a disabled recombinant herpes simplex virus type 1 (HSV-1) vector. However, before describing the methodology in detail, it is important to briefly consider the structure and life cycle of HSV1 and the modifications that must be made to the virus in order to generate an efficient gene delivery vector.

Affiliation(s): (2) Department of Immunology and Molecular Pathology, University College London, London, UK
(3) Department of Immunology and Molecular Pathology, University College London, London, UK
(4) Biovex Ltd, Oxford, UK

Book Title: Viral Vectors for Gene Therapy: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 76 | Pub. Date: Oct-07-2002 | Page Range: 33-49 | DOI: 10.1385/1-59259-304-6:33

Subject: Genetics/Genomics

References

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