SpringerProtocols: Abstract: Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification

Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification

By: Wolfgang Klein²

Abstract

Full Text

Download PDF (280K)

Protein purification is an important tool for investigations on protein function, structure analysis, and biotechnological use. Therefore a number of different techniques have been developed for fast, reliable, and reproducible overexpression and purification of relevant proteins. Affinity systems have been employed frequently due to speed, yield, and reduction of chromatographic steps necessary in order to get a highly purified protein. Over the years, different tags and matrices have been introduced to the scientific community, each providing a combination of advantages and disadvantages in the light of the protein of interest.

Affiliation(s): (2) Institute for Pharmaceutical Biology, Rheinische Friedrich-Wilhelm University Bonn, Bonn, Germany

Book Title: E. coliGene Expression Protocols

Series: Methods in Molecular Biology | Volume: 205 | Pub. Date: Oct-22-2002 | Page Range: 79-97 | DOI: 10.1385/1-59259-301-1:79

Subject: Infectious Diseases

References

Download References

1.	Vaillancourt, P., Simcox, T. G., and Zheng, C. F. (1997) Recovery of polypep-tides cleaved from purified calmodulin-binding peptide fusion proteins. Biotechniques 22, 45–453.

2.	Zheng, C. F., Simcox, T., Xu, L., and Vaillancourt, P. (1997) A new expression vector for high level protein production, one step purification, and direct isotopic labeling of calmodulin-binding peptide fusion proteins. Gene 186, 55–60.

3.	Vaillancourt, P., Zheng, C. F., Hoang, D. Q., and Breister, L. (2000) Affinity purification of recombinant proteins fused to calmodulin or to calmodulin-binding peptides. Methods Enzymol. 326, 340–362.

4.	Studier, F. W. and Moffatt, B. A. (1986) Use of bacteriophage T7 polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113–130.

5.	Carr, D. W., Stofko-Hahn, R. E., Fraser, I. D., et al. (1991) Interaction of the regulatory subunit (RII) of cAMP-dependent protein kinase with RII-anchoring proteins occurs through an amphipathic helix binding motif. J. Biol. Chem. 266, 14,188–14,192.

6.	Stofko-Hahn, R. E., Carr, D. W., and Scott, J. D. (1992) A single step purification for recombinant proteins. Characterization of a microtubule associated protein (MAP 2) fragment which associates with the type II cAMP-dependent protein kinase. FEBS Lett. 302, 274–278.

7.	Maina, C. V., Riggs, P. D., Grandea, A. G., 3rd, et al. (1988) An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene 74, 365–373.

8.	Klein, W., Winkelmann, D., Hahn, M., Weber, T., and Marahiel, M. A. (2000) Molecular characterization of the transition state regulator AbrB from Bacillus stearothermophilus. Biochim. Biophys. Acta 1493, 82–90.

9.	Aslanidis, C. and de Jong, P. J. (1990) Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 18, 6069–6074.

10.	Blanar, M. A. and Rutter, W. J. (1992) Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos. Science 256, 1014–1018.

11.	Phillips, T. A., VanBogelen, R. A., and Neidhardt, F. C. (1984) The lon gene product of Escherichia coli is a heat-shock protein. J. Bacteriol. 159, 283–287.

12.	Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubendorff, J. W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185, 60–89.

13.	Dubendorff, J. W. and Studier, F. W. (1991) Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. J. Mol. Biol. 219, 45–59.

14.	Dubendorff, J. W. and Studier, F. W. (1991) Creation of a T7 autogene. Cloning and expression of the gene for bacteriophage T7 RNA polymerase under control of its cognate promoter. J. Mol. Biol. 219, 61–68.

15.	Wyborski, D. L., Bauer, J. C., Zheng, C. F., Felts, K., and Vaillancourt, P. (1999) An Escherichia coli expression vector that allows recovery of proteins with native N-termini from purified calmodulin-binding peptide fusions. Protein Expr. Purif. 16, 1–10.

16.	Miller, J. H. (1992) A short course in bacterial genetics. A laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Labortory, Cold Spring Harbor, New York.

17.	Chung, C. T., Niemela, S. L., and Miller, R. H. (1989) One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. USA 86, 2172–2175.

18.	Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (1992) Short protocols in molecular biology. John Wiley & Sons, Harvard Medical School.

19.	Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

20.	Lämmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.

21.	Bennett, J. and Scott, K. J. (1971) Quantitative staining of fraction I protein in polyacrylamide gels using Coomassie brillant blue. Anal. Biochem. 43, 173–182.

22.	Bradford, M. M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.

23.	Schägger, H. and Jagow, G. V. (1987) Tricine-sodium dodecyl sulfate-polyacry-lamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368–379.

24.	Bloom, H., Beier, H., and Gross, H. S. (1987) Improved silver staining of plant proteins, RNA, and DNA in polyacrylamide gels. Electrophoresis 8, 93–99.

25.	Wingfield, P. T. (1995) Preparation of soluble proteins from Escherichia coli. in Current protocols inprotein science, Vol. 1 (Coligan, J. E., Dunn, B. M., Ploegh, H. L., Speicher, D. W., and Wingfield, P. T., eds.), Wiley and Sons, New York.

26.	Chong, Y. and Chen, H. (2001) Preparation of functional recombinant protein using a nondetergent sulfobetaine. BioTechniques 45, 24–26.

27.	Wingfield, P. T., Palmer, I., and Liang, S.-M. (1995) Folding and purification of insoluble (inclusion-body) proteins from Escherichia coli. in Current protocols inprotein science, Vol. 1 (Coligan, J. E., Dunn, B. M., Ploegh, H. L., Speicher, D. W., and Wingfield, P. T., eds.), Wiley and Sons, New York.

28.	Neu, H. C. and Heppel, L. A. (1965) The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J. Biol. Chem. 240, 3685–3692.

Comments (Loading...)

Post comment/View all comments