SpringerProtocols: Abstract: 11. Recording from Macropatches

11. Recording from Macropatches

By: Frances Moody-Corbett⁴, Mark Fry⁵

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The purpose of this chapter is to describe the procedures used to record macroscopic currents from patches of biological membranes. Several variations of the classic patch-clamp recording procedure are available and described in other chapters of this book, however the procedure reported here is unique from single-channel recording, loose patch-clamp recording, and giant-patch recording. Like single-channel recording and giant-patch recording, macropatch recording requires the formation of a tight seal between the recording pipet and the membrane. However, the size of the pipet tip used for macropatch recording is typically larger than that used in single-channel recording and considerably smaller than the pipets used for giant-patch recording (see Subheading 3.2.3.). It is possible using macropatch recording to determine both the identity of currents as well as their spatial distribution in biological membranes.

Affiliation(s): (4) Division of Basic Medical Sciences, Memorial University of Newfoundland, St. John’s, Canada
(5) Department of Physiology, Dartmouth Medical School, Hanover, NH

Book Title: Patch-Clamp Analysis: Advanced Techniques

Series: Neuromethods | Volume: 38 | Pub. Date: Jul-01-2002 | Page Range: 287-299 | DOI: 10.1385/1-59259-276-7:287

Subject: Neuroscience

References

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