SpringerProtocols: Abstract: 6. Perforated Patch-Clamp Technique

6. Perforated Patch-Clamp Technique

By: Raimondo D’Ambrosio⁴

Abstract

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The great advantage of patch clamp in cellular physiology is that it allows sensitive and reliable analysis of the electrical activity of cell membranes at the molecular level. Whole cell is the most popular variant of the patch-clamp technique. It is easy to obtain and it allows the use of intra- and extra-cellular recording solutions particularly devised to isolate the ionic membrane conductance of interest, or to intracellularly apply modulators or drugs. Patch clamp is therefore a very powerful technique, but the study of intracellular signaling-and the assessment of its impact on cellular electricity-requires the experimenter to perform recordings from virtually intact cells.

Affiliation(s): (4) Department of Neurosurgery, University of Washington, Seattle, WA

Book Title: Patch-Clamp Analysis: Advanced Techniques

Series: Neuromethods | Volume: 38 | Pub. Date: Jul-01-2002 | Page Range: 195-216 | DOI: 10.1385/1-59259-276-7:195

Subject: Neuroscience

References

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Abe, Y., Furukawa, K., Itoyama, Y., and Akaike, N. (1994) Glycine response in acutely dissociated ventromedial hypothalamic neuron of the rat: new approach with gramicidin perforated patch-clamp technique. J. Neurophys-iol. 72, 1530–1537.

Ahn, S. C., Lee, S. J., Goo, Y. S., Sim, J. H., So, I., and Kim, K. W. (2001) Protein kinase C suppresses spontaneous, transient, outwards K⁺currents through modulation of the Na/Ca exchanger in guinea-pig gastric myocytes. Pflugers Arch. 441(4), 417–424.

Akaike, N. and Harata, N. (1994) Nystatin perforated patch recording and its applications to analyses of intracellular mechanisms. Japn. J. Physiol. 44, 433–473.

Andersen, O. S., Durkin, J. T., and Koeppe, R. E. II. (1988) Transport through membranes: carriers, channels, and pumps (Pullman, A., et al., eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 115–132.

Bekar, L. K., Jabs, R., and Walz, W. (1999) GABAA receptor agonists modulate K+currents in adult hippocampal glial cells in situ. Glia 26(2), 129–138.

Bekar, L. K. and Walz, W. (1999) Evidence for chloride ions as intracellular messenger substances in astrocytes. J. Neurophysiol. 82(1), 248–254.

Cass, A., Finkelstein, A., and Krespi, V. (1970) The ion permeability induced in thin lipid membranes by the polyene antibiotics nystatin and amphoteri-cin B. J. Gen. Physiol. 56, 100–124.

Cass, A. and Dalmark, M. (1973) Equilibrium dialysis of ions in nystatin-treated red cells. Nature 244, 47–49.

Dean, J. B., Huang, R.-Q., Erlichman, J. S., Southard, T. L., and Hellard, D. T. (1997) Cell-cell coupling occurs in dorsal medullary neurons after minimizing anatomical-coupling artifacts. Neuroscience 80(1), 21–40.

Duchatelle-Gourdon, I., Lagrutta, A. A., and Hartzell, H. C. (1991) Effects of Mg²⁺on basal and beta-adrenergic-stimulated delayed rectifier potassium current in frog atrial myocytes. J Physiol. 435, 333–347.

Fan, J. S. and Palade, P. (1998) Perforated patch recordings with β-escin. Plügers Arch. 436, 1021–1023.

Hille, B. (1992) Ionic Channels of Excitable Membranes, 2nd ed. Sinauer, Sunder land, MA.

Horn, R. and Marty, A. (1988) Muscarinic activation of ionic currents measured by a new whole-cell recording method. J. Gen. Physiol. 92, 145–159.

Horn, R. (1991) Diffusion of nystatin in plasma membrane is inhibited by a glass-membrane seal. Biophys. J. 60, 329–333.

Johnson, B. D. and Byerly, L. (1993) A cytoskeletal mechanism for Ca²⁺channel metabolic dependence and inactivation by intracellular Ca²⁺. Neuron 10(5)

Johnson, B. D. and Byerly, L. (1994) Ca²⁺channel Ca(²⁺)-dependent inactivation in a mammalian central neuron involves the cytoskeleton. Pflugers Arch. 429(1), 14–21.

Kobayashi, S., Kitazawa, T., Somlyo, A. V., and Somlyo, A. P. (1989) Cytosolic heparin inhibits muscarinic and β-adrenergic Ca²⁺release in smooth muscle. J. Biol. Chem. 264, 17,997–18,004.

Konishi, M. and Watanabe, M. (1995) Molecular size-dependent leakage of intra cellular molecules from frog skeletal muscle fiber permeabilized with β-escin. Plügers Arch. 429, 598–600.

Korn, S. J. and Horn, R. (1989) Influence of sodium-calcium exchange on calcium currents rundown and the duration of calcium-dependent chloride currents in pituitary cells, studies with whole cell and perforated patch recording. J. Gen. Physiol. 94, 789–812.

Kyrozis, A. and Reichling, D. B. (1995) Perforated-patch recording with gramicidin avoids artifactual changes in intracellular chloride concentration. J. Neurosci. Methods 57, 27–35.

Lampen, J. O. (1966) Interference of polyenic antifungal antibiotics (especially nystatin and filipin) with specific membrane function, in Biochemical Studies of Antimicrobial Drugs (Newton, B. A. and Reynolds, P. E., eds.), The Society for General Microbiology, Cambridge, MA, p. 111.

Levitan, E. S. and Kramer, R. H. (1990) Neuropeptide modulation of single calcium and potassium channels detected with a new patch clamp configuration. Nature 348, 545–547.

Lindau, M. and Fernandez, J. M. (1986) IgE-mediated degranulation of mast cells does not require opening of ion channels. Nature 319(6049), 150–153.

Lopantsev, V. and Schwartzkroin, P. A. (1999) GABAA-Dependent chloride influx modulates GABAB-mediated IPSPs in hippocampal pyramidal cells. J. Neurophysiol. 82(3), 1218–1223.

Magistretti, J., Mantegazza, M., Guatteo, E., and Wanke, E. (1996) Action potentials recorded with patch-clamp amplifiers: are they genuine? Trends Neurosci. 19(12), 530–534.

Marty, A. and Finkelstein, A. (1975) Pores formed in lipid bilayer membranes by nystatin. J. Gen. Physiol. 65, 515–526.

Mazzanti, M., Assandri, R., Ferroni, A., and DiFrancesco, D. (1996) Cytoskeletal control of rectification and expression of four substates in cardiac inward rectifier K⁺channels. FASEB J. 10(2), 357–361.

McKhann, G. M. II, D’Ambrosio, R., and Janigro, D. (1997) Heterogeneity of astrocyte resting membrane potentials and intercellular coupling revealed by whole-cell and gramicidin-perforated patch recordings from cultured neocortical and hippocampal slice astrocytes. J.Neurosci. 17(18), 6850–6863.

Muraki, K., Imaizumi, Y., and Watanabe, M. (1992) Ca-dependent K channels in smooth muscle cells permeabilized by beta-escin recorded using the cell attached patch-clamp technique. Pflugers Arch. 420(5-6), 461–469.

Rae, J., Cooper, K., Gates, P., and Watsky, M. (1991) Low access resistance perforated patch recordings using anphotericin B. J. Neurosci. Methods 37, 15–26.

Rhee, J.-S., Ebihara, S., and Akaike, N. (1994) Gramicidin perforated patch-clamp technique reveals glycine-gated outward chloride current in dissociated nucleus solitarii neurons of the rat. J. Neurophysiol. 72, 1103–1108.

Sawyer, D. B., Koeppe, R. E., and Andersen, O. S. (1989) Induction of conductance heterogeneity in gramicidin channels. Biochemistry 28, 6571–6583.

Tajima, Y., Ono, K., and Akaike, N. (1996) Perforated patch-clamp recording in cardiac myocytes using cation-selective ionophore gramicidin. Am. J. Physiol. 271(2 Pt 1), C524–C532.

Vandeputte, J., Wachtel, J. L., and Stiller, E. T. (1956) Amphotericins A and B, antifungal antibiotics produced by streptomyces. II. The isolation and properties of the crystalline amphotericins, in Antibiotics Annual, 1955-1956. Medical Encyclopedia, Inc., New York, pp.587–591.

Wanke, E., Ferroni, A., Malgaroli, A., Ambrosini, A., Pozzan, T., and Meldolesi, J. (1987) Activation of a muscarinic receptor selectively inhibits a rapidly inactivated Ca²⁺current in rat sympathetic neurons. Proc. Natl. Acad. Sci. USA 84(12), 4313–4317.

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