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Southern Blotting of Agarose Gels by Capillary Transfer
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The detection of specific nucleic acid species following electrophoretic separation of a complex sample may be undertaken
by the use of Southern blotting (1). Genomic DNA fragments are separated according to size by agarose gel electrophoresis following digestion with suitable
restriction enzymes (see Chapter 5).
Affiliation(s): (3) Department of Biosciences, University of Hertfordshire, Hatfield, UK
(4) Department of Haematology, Birmingham Children’s Hospital NHS Trust, Birmingham, UK
(4) Department of Haematology, Birmingham Children’s Hospital NHS Trust, Birmingham, UK
Book Title: PCR Mutation Detection Protocols
Series: Methods in Molecular Biology | Volume: 187 | Pub. Date: Apr-04-2002 | Page Range: 23-27 | DOI: 10.1385/1-59259-273-2:023
Subject: Genetics/Genomics
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