Contents of this article

Useful Tools
New Culture
Abstract
As the title indicates, this culture method was developed by Denis New and first described in 1955 (1). It enables the observer to study the events of gastrulation in the chick embryo in much greater detail than possible until then. It also opens the way to microsurgery without the problem that yolk and the vitelline membrane cause. Studying the effects of treatment with compound affecting morphogenesis and development became meaningful, because precise concentrations and volumes could be administered and successfully washed out again.
Affiliation(s): (2) MRC Brain Development Programme, Department of Developmental Neurobiology, Guy’s Campus, King’s College, London, UK
Series: Methods in Molecular Biology  |  Volume: 97  |  Pub. Date: Jul-01-1999  |  Page Range: 225-243  |  DOI: 10.1385/1-59259-270-8:225
Subject:  Cell Biology
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