SpringerProtocols: Abstract: Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells

Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells

By: Anna-Katerina Hadjantonakis², Melinda Pirity², András Nagy²

Abstract

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The introduction and establishment of transgenic, and in particular embryonic stem (ES) cell-based gene “knockout” technologies have made the mouse a key player in studying embryonic development and disease (1,2). In recent years, methods for the production of more complex genomic alterations have become increasingly widespread, hinting at an ability to manipulate and study a mammalian genome to an extent never previously thought possible. Such methodologies often partner homologous recombination-mediated gene targeting or random integration with site-specific recombination events.

Affiliation(s): (2) Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada

Book Title: Molecular Embryology: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 97 | Pub. Date: Jul-01-1999 | Page Range: 101-122 | DOI: 10.1385/1-59259-270-8:101

Subject: Cell Biology

References

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