SpringerProtocols: Abstract: NMR of Flavoproteins

NMR of Flavoproteins

By: Jacques Vervoort², Marco Hefti²

Abstract

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Flavoproteins are involved in a wide variety of enzymatic reactions like oxidation, reduction and mono-oxygenation. Consequently, flavin cofactors occur in many different forms including, for example, the oxidized semiquinone and reduced states as well as the C4a-(hydro)peroxyflavin form (Fig. 1 and Fig. 2). In flavoprotein research the geometrical and electronic properties of all these different flavin species are of interest. This chapter deals with the use of nuclear magnetic resonance (NMR), a technique enabling the determination of detailed structural and electronic characteristics of flavoproteins and their cofactors.

Affiliation(s): (2) Department of Biomolecular Sciences, Wageningen Agricultural University, Wageningen, The Netherlands

Book Title: Flavoprotein Protocols

Series: Methods in Molecular Biology | Volume: 131 | Pub. Date: Jul-01-1999 | Page Range: 139-147 | DOI: 10.1385/1-59259-266-X:139

Subject: Protein Science

References

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