SpringerProtocols: Abstract: Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction

Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction

By: Alfred N. Fonteh, William Reed, James M. Samet

Abstract

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The release of arachidonic acid (AA) from membrane phospholipids by phospholipase A₂ (PLA₂) is a critical step in the generation of lipid mediators by mast cells. AA released by PLA₂ activity is metabolized to bioactive compounds, such as prostaglandins, thromboxane, and leukotrienes. During the last decade, several mammalian PLA2s have been shown to play a role in the mobilization of AA from phospholipids. At least ten different PLA₂ types have been described (1,2). In general, these consist of secretory, cytosolic, or membrane bound activities.

Book Title: Eicosanoid Protocols

Series: Methods in Molecular Biology | Volume: 120 | Pub. Date: Mar-25-1999 | Page Range: 91-105 | DOI: 10.1385/1-59259-263-5:91

Subject: Biochemistry

References

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