SpringerProtocols: Abstract: Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry

Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry

By: M. Kerry O’Banion, John A. Olschowka

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Prostaglandin production is dependent on the presence of an enzyme called prostaglandin G/H synthase, better known as cyclooxygenase, that catalyzes the first steps in the conversion from arachidonic acid. A cDNA for cyclooxygenase was first cloned from sheep seminal vesicles in 1988 (1,2). In 1991, we and others reported the cloning of a second isoform of cyclooxygenase, now known as COX-2, that is rapidly induced in response to growth factors and proinflammatory cytokines and downregulated by glucocorticoid hormones (3–6).

Book Title: Eicosanoid Protocols

Series: Methods in Molecular Biology | Volume: 120 | Pub. Date: Mar-25-1999 | Page Range: 55-66 | DOI: 10.1385/1-59259-263-5:55

Subject: Biochemistry

References

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