SpringerProtocols: Abstract: 6. Purification and Use of DNA Minicircles with Different Linking Numbers

6. Purification and Use of DNA Minicircles with Different Linking Numbers

By: Giorgio Camilloni³, Rodolfo Negri⁴, Micaela Caserta⁵, Ernesto Di Mauro³

Abstract

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The structural organization of both prokaryotic and eukaryotic chromosomes has evolved following a common principle: the need for storing the genetic information in topologically independent domains consisting of one or more genes and of all the elements required in cis for their functioning. The elements that belong to the same functional unit are topologically linked: they depend on each other and cooperate.

Affiliation(s): (3) Dipartimento di Genetica e Biologia Molecolare, Universitd di Roma, La Sapienza, Roma, Italy
(4) Centro di Studio per gli Acidi Nucleici (CNR), Dipartimento di Genetica e Biologia Molecolare, Universitá di Roma, La Sapienza, Rome, Italy
(5) Centro di Studio per gli Acidi Nucleici (CNR), Dipartimento di Genetica e Biologia Molecolare, Universitd di Roma, La Sapienza, Rome, Italy

Book Title: DNA Topoisomerase Protocols: Volume I: DNA Topology and Enzymes

Series: Methods in Molecular Biology | Volume: 94 | Pub. Date: Mar-23-1999 | Page Range: 51-60 | DOI: 10.1385/1-59259-259-7:51

Subject: Genetics/Genomics

References

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