SpringerProtocols: Abstract: 3. Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomers

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3. Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomers

By: Ryo Hanai³, Joaquim Roca⁴

Abstract

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Gel electrophoresis in one dimension is often insufficient to distinguish various molecular species of DNA, since different conformers, and sometimes DNA molecules of totally different structures may have the same electrophoretic mobility. These DNA species can be resolved by two-dimensional (2-D) gel electrophoresis, which involves two successive operations carried out with one gel slab under different conditions and in orthogonal directions.

Affiliation(s): (3) Department of Chemistry, Rikkyo University, Tokyo, Japan
(4) Departament de Biologia Molecular i Cellular, Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Cientificas, Barcelona, Spain

Book Title: DNA Topoisomerase Protocols: Volume I: DNA Topology and Enzymes

Series: Methods in Molecular Biology | Volume: 94 | Pub. Date: Mar-23-1999 | Page Range: 19-27 | DOI: 10.1385/1-59259-259-7:19

Subject: Genetics/Genomics

References

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