Springer Protocols: Abstract: Induction of Intracellular Signals Through Binding of Adhesive Molecules: Activation of p125FAK Tyrosine Phosphorylation

Induction of Intracellular Signals Through Binding of Adhesive Molecules: Activation of p125FAK Tyrosine Phosphorylation

By: Mascia Venturino², Cristina Olivo², Guido Tarone², Paola Defilippi²

Abstract

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p125FAK tyrosine phosphorylation is a primary response to integrin stimulation. The first evidence that integrin occupancy by ligands induces tyrosine phosphorylation came from studies on the fibrinogen receptor αllbβ3 in platelets (1). Further work showed that both in normal and transformed cells several different integrin receptors can trigger tyrosine phosphorylation of intracellular proteins following interactions with matrix proteins or integrin receptor clustering by specific antibodies (2).

Affiliation(s): (2) Sezione di Biologia, Dipartimento di Genetica, Biologia e Chimica Medica, Università di Torino, Torino, Italy

Book Title: Adhesion Protein Protocols

Series: Methods in Molecular Biology | Volume: 96 | Pub. Date: Feb-01-1999 | Page Range: 191-198 | DOI: 10.1385/1-59259-258-9:191

Subject: Cell Biology

References

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1.	Ferrel. J. E. and Martin, G. S. (1989) Tyrosine-specific protein phosphorylation is regulated by glycoprotein llb-llla in platelets. Proc. Natl. Acad. Sci. USA 86, 2234–2238.

2.	Clark, E. A. and Brugge, J. S. (1995) Integrins and signal transduction pathways: the road taken. Science 8, 233–239.

3.	Kornberg, L., Earp, H. S., Parsons, J. T., Schaller, M., and Juliano R. L. (1992) Cell adhesion of integrin clustering increases phosphorylation of a focal adhesion associated tyrosine kinase. J. Biol. Chem. 267, 23,439–23,442.

4.	Burridge, C. A., Turner, C., and Romer, L. (1992) Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell. Biol. 119, 893–904.

5.	Clark, E. A., Shattil, S. J., Ginsberg, M. H., Bolen, J., and Brugge, J. S. (1994) Regulation of the protein tyrosine kinase pp72syk by platelet agonists and the integrin alphallb/beta3. J. Biol. Chem. 269, 28,859–28,864.

6.	Sakai, R., Iwamatsu, A., Hirano, N., Ogawa, S., Tanaka, T., Mano, H., Yazaki, Y., and Hirai, H. (1994) A novel signaling molecule, p130, forms stable complexes in vivo with v-crk and c-src in a tyrosine phosphorylation-dependent manner. EMBO J. 13, 3748–3756.

7.	Nojima, Y., Tachibana, K., Sato, T., Schlossman, S. F., and Morimoto, C. (1995) Focal adhesion kinase is tyrosine phosphorylated after engagement of α4/β1 and α5/β1 integrins on human T-lymphoblastic cells. Cell Immunol. 161, 8–13.

8.	Vuori, K. and Ruoslahti, E. (1995) Tyrosine phosphorylation of p130cas and cortactin accompanies integrin-mediated cell adhesion to extracellular matrix. J. Biol. Chem. 270, 22,259–22,262.

9.	Hanks, S. K., Calalb, M. B., Harper, M. C., and Patel, S. K. (1992) Focal adhesion proteintyrosine kinase phosphorylated in response to cell attachment to fibronectin. Proc. Natl. Acad. Sci. USA 89, 8487–8491.

10.	Schaller M. D., Borgman, C. A., Cobb. B. S., Vines, R. R., Reynolds, A. B. Parsons. J. T. (1992) pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Proc. Natl. Acad. Sci. USA 89, 5192–5196.

11.	Defilippi, P., van Hinsberg, V., Bertolotto, A., Rossino, P., Silengo, L., and Tarone, G. (1991) Differential distribution and modulation of expression of alpha1/beta1 integrin on human endothelial cells. J. Cell. Biol. 114, 855–863.

12.	Ey, P. L., Prowse, S. J., and Jenkin, C. R. (1978) Isolation of pure IgG1 and IgG2b immunoglobulins from mouse serum using Protein A-Sepharose. Immunochem. 15, 429–436.

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