Springer Protocols: Abstract: Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules: Selective Protocols of Immunization and Screening Assays for Generation of Blocking, Activating and Activation Reporter Antibodies

Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules: Selective Protocols of Immunization and Screening Assays for Generation of Blocking, Activating and Activation Reporter Antibodies

By: Carlos Cabañas², Francisco Sánchez-Madrid³

Abstract

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The specificity, homogeneity, and ability to be produced in unlimited amounts have made monoclonal antibodies (MAbs) an extremely useful tool for the study of a great variety of molecules involved in cellular adhesion phenomena. In many cases, the detailed biochemical and functional characterization of members of the integrin, selectin, immunoglobulin, and cadherin families of adhesion receptors, and their specific cellular and tissue distribution have only been made possible through the development and use of specific MAbs to these molecules.

Affiliation(s): (2) Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain
(3) Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, Madrid, Spain

Book Title: Adhesion Protein Protocols

Series: Methods in Molecular Biology | Volume: 96 | Pub. Date: Feb-01-1999 | Page Range: 1-9 | DOI: 10.1385/1-59259-258-9:1

Subject: Cell Biology

References

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1.	Frelinger, A. L., III, Du, X., Plow, E. F., and Ginsberg, M. H. (1991) Monoclonal antibodies to ligand-occupied conformers of integrin αIIbβ3 after receptor affinity, specificity and function. J. Biol. Chem. 266, 17,106–17,111.

2.	Faull, R. J., Kovach, N. L., Harlan, J. M., and Ginsberg, M. H. (1994) Stimulation of integrin-mediated adhesion of T lymphocytes and monocytes: Two mechanisms with divergent biological consequences. J. Exp. Med. 179, 1307–1316.

3.	Takada, Y. and Puzon, W. (1993) Identification of a regulatory region of integrin b1 subunit using activating and inhibiting antibodies. J. Biol. Chem. 268, 17,597–17,601.

4.	Dransfield, I., Cabañas, C., Craig, A., and Hogg, N. (1992) Divalent cation regulation of the function of the leukocyte integrin LFA-1. J. Cell Biol. 116, 219–226.

5.	Mould, A. P., Garratt, A. N., Askari, J. A., Akiyama, S. K., and Humphries, M. J. (1995). Identification of a novel anti-integrin monoclonal antibody that recognizes a ligand-induced binding site epitope on the b1 subunit. FEBS Lett. 363, 118–122.

6.	Arroyo, A. G., García-Pardo, A., and Sánchez-Madrid, F. (1993) A high affinity conformational state on VLA integrin heterodimers induced by an anti-β1 chain monoclonal antibody. J. Biol. Chem. 268, 9863–9868.

7.	Luque, A., Gómez, M., Puzon, W., Takada, Y., Sánchez-Madrid, F., and Cabañas, C. (1996) Activated conformations of Very Late Activation integrins detected by a group of antibodies (HUTS) specific for a novel regulatory region (355–425) of the common β1 chain. J. Biol. Chem. 271, 11,067–11,075.

8.	Hemler, M. E., Sánchez-Madrid, F., Flotte, T. J., Krensky, A. M., Burakoff, S. J., Bhan, A. K., Springer, T. A., and Strominger, J. L. (1984) Glycoproteins of 210. 000 and 130. 000 m. w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines. J. Immunol. 132, 3011–3018.

9.	Sánchez-Madrid, F., de Landázuri, M. O., Morago, G., Cebrián, M., Acevedo, A., and Bernabeu, C. (1986) VLA-3: a novel polypeptide association within the VLA molecular complex: cell distribution and biochemical characterization. Eur. J. Immunol. 16, 1343–1349.

10.	Cabañas, C., Sánchez-Madrid, F., Bellýn, T., Figdor, C. G., Te Velde, A. A., Fernández, J. M., Acevedo, A., and Bernabeu, C. (1989) Characterization of a novel myeloid antigen regulated during differentiation of monocytic cells. Eur. J. Immunol. 19, 1373–1378.

11.	Galfré, G. and Milstein, C. (1981) Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 73, 3.

12.	Brown, G. and Ling, N. R. (1988) Murine monoclonal antibodies, in Antibodies, vol I. A practical approach (Catty, D., ed.) IRL, Oxford.

13.	Harlow, E. and Lane, D. (1988) Antibodies, a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

14.	Hockfield, S., Carlson, S., Evans, C., Levitt, P., Pintar, J., and Siberstein, L. (1993) Selected methods for antibody and nucleic acid probes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

15.	Gýmez, M., Luque, A., del Pozo, M. A., Sánchez-Madrid, F., and Cabañas, C. (1997) Functional relevance during lymphocyte migration and cellular localization of a ligand-induced binding site on β1 integrins. Eur. J. Immunol., 27, 8–10.

16.	Keizer, G. D., Visser, W., Vliem, M., and Figdor, C. G. (1988) A monoclonal antibody (NKI-L16) directed against a unique epitope on the a-chain of human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions. J. Immunol. 140, 1393–1400.

17.	Campanero, M. R., Pulido, R., Ursa, M. A., Rodriguez-Moya, M., de Landázuri, M. O., and Sánchez-Madrid, F. (1990) An alternative leukocyte homotypic adhesion mechanism, LFA-1/ICAM-1 independent, triggered through the human VLA-4 integrin. J. Cell Biol. 110, 2157–2165.

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