SpringerProtocols: Abstract: Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System

Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System

By: Takumi Era², Stephane Wong², Owen N. Witte²

Abstract

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Numerous causative genes involved in human cancers, such as leukemias and lymphomas, have been identified (1). Considerable evidence has accumulated showing that disruptions of these genes can affect hematopoietic cell differentiation and growth. However, it remains largely unknown how these molecules function directly in hematopoietic stem cells.

Affiliation(s): (2) Howard Hughes Medical Institute, University of California, Los Angeles, CA

Book Title: Embryonic Stem Cells: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 185 | Pub. Date: Nov-12-2001 | Page Range: 83-95 | DOI: 10.1385/1-59259-241-4:83

Subject: Cell Biology

References

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1.	Lowenberg, B., Downing, J. R., and Burnett, A. (1999) Acute myeloid leukemia. N. Engl. J. Med. 341, 1051–1062.

2.	Sawyers, C. L. (1999) Chronic myeloid leukemia. N. Engl. J. Med. 340, 1330–1340.

3.	Groffen, J., Stephenson, J. R., Heisterkamp, N., de Klein, A., Bartram, C. R., and Grosveld, G. (1984) Philadelphia chromosomal breakpoints are clustered within a limited region, bcr, on chromosome 22. Cell 36, 93–99.

4.	Konopka, J. B., Watanabe, S. M., and Witte, O. N. (1984) An alteration of the human c-abl protein in K562 leukemia cells unmasks associated tyrosine kinase activity. Cell 37, 1035–1042.

5.	Shtivelman, E., Lifshitz, B., Gale, R. P., Roe, B. A., and Canaani, E. (1985) Fused transcript of abl and bcr genes in chronic myelogenous leukaemia. Nature 315, 550–554.

6.	McLaughlin, J., Chianese, E., and Witte, O. N. (1987) In vitro transformation of immature hematopoietic cells by the P210 BCR/Abl oncogene product of the Philadelphia chromosome. Proc. Natl. Acad. Sci. USA 84, 6558–6562.

7.	Lugo, T. G., Pendergast, A., Muller, A. J., and Witte, O. N. (1990) Tyrosine kinase activity and transformation potency of bcr-abl oncogene products. Science 247, 1079–1082.

8.	Daley, G. Q. and Baltimore, D. (1988) Transformation of an interleukin 3-dependent hematopoietic cell line by the chronic myelogenous leukemia-specific P210+bcr/abl~ protein. Proc. Natl. Acad. Sci. USA 85, 9312–93

9.	Gishizky, M. L. and Witte, O. N. (1992) Initiation of deregulated growth of multipotent progenitor cells by bcr-abl in vitro. Science 256, 836–839.

10.	Daley, G. Q., Van Etten, R. A., and Baltimore, D. (1990) Induction of chronic myelogenous leukemia in mice by the P210^bcr/abl gene of the Philadelphia chromosome. Science 247, 824–830.

11.	Kelliher, M. A., McLaughlin, J., Witte, O. N., and Rosenberg, N. (1990) Induction of a chronic myelogenous leukemia-like syndrome in mice with v-abl and BCR/ABL. Proc. Natl. Acad. Sci. USA 87, 6649–6653.

12.	Honda, H., Oda, H., Suzuki, T., Takahashi, T., Witte, O. N., Ozawa, K., et al. (1998) Development of acute lymphoblastic leukemia and myeloproliferative disorder in transgenic mice expressing p210bcr/abl: a novel transgenic model for human Ph1-positive leukemias. Blood 91, 2067–2075.

13.	Pear, W. S., Miller, J. P., Xu, L., Pui, J. C., Soffer, B., Quackenbush, R. C., et al. (1998) Efficient and rapid induction of a chronic myelogenous leukemia-like myeloproliferative disease in mice receiving P210 bcr/abl-transduced bone marrow. Blood 92, 3780–3792.

14.	Nakano, T., Kodama, H., and Honjo, T. (1994) Generation of lymphohematopoietic cells from embryonic stem cells in culture. Science 265, 1098–1101.

15.	Nakano, T. and Kodama, H. H. (1996) In vitro development of primitive and definitive erythrocytes from different precursors. Science 272, 722–724.

16.	Era, T., Takahashi, T., Sakai, K., Kawamura, K., and Nakano, T. (1997) Thrombopoietin enhances proliferation and differentiation of murine yolk sac erythroid progenitors. Blood 89, 1207–1213.

17.	Era, T., Takagi, T., Takahashi, T., Bories, J. C., and Nakano, T. (2000) Characterization of hematopoietic lineage-specific gene expression by ES cell in vitro differentiation induction system. Blood 95, 870–878.

18.	Gossen, M. and Bujard, H. (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 89, 5547–5551.

19.	Era, T. and Witte, O. N. (2000) Regulated expression of P210 Bcr-Abl during embryonic stem cell differentiation stimulates multipotential progenitor expansion and myeloid cell fate. Proc. Natl. Acad. Sci. USA 97, 1737–1742.

20.	Baron, U., Gossen, M., and Bujard, H. (1997) Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential. Nucleic Acids Res. 25, 2723–2729.

21.	Niwa, H., Burdon, T., Chambers, I., and Smith, A. (1998) Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3. Genes Dev. 12, 2048–2060.

22.	Niwa, H., Yamamura, K., and Miyazaki, J. (1991) Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108, 193–199.

23.	Robertson, E. J. (1987) Embryo-derived stem cells, in Teratocacinomas and Embryonic Stem Cell: A Practical Approach (Robertson, E. J., ed.), IRL Press, New York, pp. 71–112.

24.	Wurst, W. and Joyner, A. L. (1993) Gene Targeting: A Practical Approach, vol. 126. IRL Press, New York.

25.	Chambard, J. C. and Pognonec, P. (1998) A reliable way of obtaining stable inducible clones. Nucleic Acids Res. 26, 3443–3444.

26.	Ramirez-Solis, R., Davis, A. C., and Bradley, A. (1993) Gene targeting in embryonic stem cells. Methods Enzymol. 225, 855–878.

27.	Cho, S. K., Webber, T. D., Carlyle, J. R., Nakano, T., Lewis, S. M., and Zuniga-Pflucker, J. C. (1999) Functional characterization of B lymphocytes generated in vitro from embryonic stem cells. Proc. Natl. Acad. Sci. USA 96, 9797–9802.

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