SpringerProtocols: Abstract: Mapping of Genomic Clones by Fluorescence In Situ Hybridization

Mapping of Genomic Clones by Fluorescence In Situ Hybridization

By: Margaret A. Leversha²

Abstract

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Fluorescence in situ hybridization (FISH) provides a rapid means of placing labeled DNA segments into a wider genomic context. Mapping to banded metaphase chromosomes anchors clones for specific genes or markers in a well-recognized framework and provides a useful confirmation that clones belong to the expected region of interest.

Affiliation(s): (2) Roy Castle International Centre for Lung Cancer Research, Liverpool, UK

Book Title: Genomics Protocols

Series: Methods in Molecular Biology | Volume: 175 | Pub. Date: Jun-10-2001 | Page Range: 109-127 | DOI: 10.1385/1-59259-235-X:109

Subject: Genetics/Genomics

References

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