SpringerProtocols: Abstract: 12. Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study

12. Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study

By: M.A. Nasser Hajibagheri², Kenneth Sawin³, Steve Gschmeissner², Ken Blight², Carol Upton²

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The fission yeast Schizosaccharomyces pombe serves as a model system for investigating a wide variety of problems in eukaryotic cellular and molecular biology. Although probably most widely studied in relation to the control of the eukaryotic cell cycle (1) and the events of cell division (2,3), S. pombe has also received significant attention in studies of protein trafficking and secretion (4), cell wall biosynthesis (5), cellular and molecular aspects of meiosis, genetic recombination, and spore formation (6), signal transduction pathways and control of gene expression (7-10) and cell growth (11,12), cell morphology (13,14) and the cytoskeleton (15,16). In many ways we can think of S. pombe as essentially a typical eukaryote trapped inside a cell wall, and the resulting constraints on cell morphology confer on it a stereotyped cellular architecture, well suited to ultrastructural analysis. The details of this architecture at both the light- and electron-microscopic level can be found in reference (17); here we will only highlight certain features.

Affiliation(s): (2) Electron Microscopy Unit, Imperial Cancer Research Fund, London
(3) Cell Cycle Laboratory, Imperial Cancer Research Fund, London

Book Title: Electron Microscopy Methods and Protocols

Series: Methods in Molecular Biology | Volume: 117 | Pub. Date: Mar-09-1999 | Page Range: 183-207 | DOI: 10.1385/1-59259-201-5:183

Subject: Imaging/Radiology

References

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