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Relative Gene Expression in Normal and Tumor Tissue by Quantitative RT-PCR
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The polymerase chain reaction (PCR) is a very powerful technique for the in vitro amplification of nucleic acid sequences
(1) PCR relies on the principle that oligonucleotide sequences hybridize to a template DNA specifically, given the appropriate
conditions. Conditions such as ionic strength and temperature, when optimized, would allow two oligonucleotide primers to
hybridize to a DNA template. The primers are complementary to opposite strands of DNA, which allows DNA synthesis by a thermostable
DNA polymerase upon hybridization.
Affiliation(s): (2) USC Norris Cancer Center, Los Angeles, CA
(3) National Cancer Institute-Medicine Branch, National Naval Medical Center, Bethesda, MD
(3) National Cancer Institute-Medicine Branch, National Naval Medical Center, Bethesda, MD
Book Title: Telomeres and Telomerase: Methods and Protocols
Series: Methods in Molecular Biology | Volume: 191 | Pub. Date: Mar-01-2002 | Page Range: 83-98 | DOI: 10.1385/1-59259-189-2:83
Subject: Genetics/Genomics
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