Nonradioactive Detection of Telomerase Activity Using a PCR-ELISA-Based Telomeric Repeat Amplification Protocol
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Numerous studies on telomerase expression have consistently demonstrated the presence of telomerase activity in the vast majority
of different types of cancer, as well as immortalized cells, but have failed to detect telomerase in most normal tissues (1). Examples include breast cancer, neuroblastoma, and cervical cancers, in which a correlation between telomerase expression
and staging or clinical outcome has been demonstrated (2). Therefore, its very restricted, tumor-specific expression pattern makes telomerase an attractive target for diagnostic
and prognostic purposes and for therapeutic intervention in a variety of human cancers
Affiliation(s): (2) Roche Applied Science of Roche Diagnostics GmbH, Research Center Penzberg, Penzberg, Germany
(3) Roche Applied Science of Roche Diagnostics GmbH, Research Center Penzberg, Penzberg, Germany
(3) Roche Applied Science of Roche Diagnostics GmbH, Research Center Penzberg, Penzberg, Germany
Book Title: Telomeres and Telomerase: Methods and Protocols
Series: Methods in Molecular Biology | Volume: 191 | Pub. Date: Mar-01-2002 | Page Range: 147-158 | DOI: 10.1385/1-59259-189-2:147
Subject: Genetics/Genomics
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