SpringerProtocols: Abstract: Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons

Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons

By: Barbara Karten², Bronwyn L. MacInnis³, Hubert Eng³, Yoko Azumaya³, Grace Martin³, Karen Lund³, Russell C. Watts³, Jean E. Vance³, Dennis E. Vance³, Robert B. Campenot³

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The compartmented culture model utilizing rat sympathetic neurons and sensory neurons is gaining wide use in investigations of neurotrophic factor signaling and axonal transport. Previous chapters have focused on the preparation of compartmented cultures. Here we focus on analytical approaches that we have used with compartmented cultures that can be used to investigate apoptosis, intracellular signaling, and other issues. Techniques used for incubating the cultures with radioisotopes and other agents, harvesting cell extracts from the cultures, using the cultures for histochemistry, and producing cultures from individual transgenic mouse pups are described.

Affiliation(s): (2) Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
(3) Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada

Book Title: Apoptosis Techniques and Protocols

Series: Neuromethods | Volume: 37 | Pub. Date: Feb-12-2002 | Page Range: 163-175 | DOI: 10.1385/1-59259-188-4:163

Subject: Neuroscience

References

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