SpringerProtocols: Abstract: Deconvolution of Calcium-Binding Curves: Facts and Fantasies

Deconvolution of Calcium-Binding Curves: Facts and Fantasies

By: Jacques Haiech², Marie-Claude Kilhoffer²

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Ca²⁺signaling is of paramount importance in intracellular communication of eukaryotic cells. Many external stimuli trigger a transient change in the cytosolic-free Ca²⁺concentration (in the form of a Ca²⁺wave or Ca²⁺oscillations). The internal Ca²⁺modulation is deciphered by Ca²⁺-binding proteins, which undergo conformational changes upon Ca²⁺-binding allowing them to act as enzymatic or protein modulators. These Ca²⁺-binding proteins have been well described in the past three decades (1–6). Calmodulin, an ubiquitous and multifunctional protein, is considered as the prototype of the Ca²⁺-binding protein family containing EF-hand domains (7–16). Because of its pivotal role in many Ca²⁺-dependent cellular events, the understanding of the mechanism of action of this protein at the molecular level has been the aim of several research groups. For such a study, four main points have to be tackled:

–	Description of the mechanism of Ca²⁺-binding to calmodulin;
–	Understanding of the conformational changes induced by Ca²⁺-binding;
–	Analysis of the interaction of calmodulin with the different targets; and
–	Deciphering the activation or the modulation of the calmodulin/target protein complexes.

Affiliation(s): (2) Pharmacologie et Physico-Chimie des Interactions Cellulaires et Moléculaires, Faculté de Pharmacie, Université Louis Pasteur, Strasbourg, Illkirch, France

Book Title: Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques

Series: Methods in Molecular Biology | Volume: 173 | Pub. Date: Jan-24-2002 | Page Range: 25-42 | DOI: 10.1385/1-59259-184-1:025

Subject: Protein Science

References

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